SOVREMENNAYA PEDIATRIYA.2016.2(74):63-65; doi 10.15574/SP.2016.74.63 

Experimental studies of the effect of «SEPTOLETE» preparation on the oral cavity immunity factors 

Melnikov O. F., Prilutskaya A. D., Timchenko M. D.

SI «Institute of Otolaryngology named after Prof. A.S. Kolomiychenko NAMS of Ukraine» 

Experimental invitro studies in 120 samples in condition of contact with «Septolete» preparation (with different flavors, including Septolete plus in the form of lozenges and spray) with unstimulated oropharyngeal secretion and blood serum of healthy donors are conducted. Invivo studies were conducted in five healthy donors with the aim of determination of the humoral factors of protection of oropharyngeal secretion before and after one and two hours after application of «Septolete plus» oral lozenges. The results of studies allow to suggest that the «Septolete plus» preparation produced by KRKA d.d. Company, Slovenia, is not affected in invitro conditions on the contact of secretory IgA, other classes of serum immunoglobulins and does not alter the concentration of interferons α and γ. During the invivo studies was found that after the hour of application of «Septolete plus» lozenges was noted an increase in the content of secretory forms of IgA. 

Key words: experimental studies, oropharyngeal secret, oral cavity immunity factors, Septolete. 

REFERENCES

1. Gubler EV. 1978. Matematicheskie metodyi analiza i raspoznavaniya patologicheskih protsessov. Leningrad, Meditsina: 242.

2. Demyanov AV, Kotov AYu, Simbirtsev AG. 2003. Diagnosticheskaya tsennost issledovaniy urovnya tsitokinov v klinicheskoy praktike. Tsitokinyi i vospalenie. 3: 20—28.

3. Zabolotnyiy DI, Melnikov OF, Timchenko SV, Zabolotna DD. 2008. DoslIdzhennya rotoglotkovogo sekretu u hvorih na hronIchnI zapalnI ta alergIchnI zahvoryuvannya verhnih dihalnih shlyahIv. Metod rekom. Kiev: 28.

4. Kaydashev IP. 2003. Metodi klInIchnih ta eksperimentalnih doslIdzhen v meditsinI. Poltava, Polimet: 320.

5. Melnikov OF, Zayats TA. 1999. Sravnitelnaya otsenka radioizotopnogo i spektrofotometricheskogo metodov registratsii tsitoliza. Lab diagnostika. 2: 32—34.

6. Melnikov OF, Zayats TA. 1999. Sravnitelnaya otsenka radioizotopnogo i spektrofotometricheskogo metodov registratsii tsitoliza. Lab diagnostika: 294.

7. Melnikov OF, Timchenko MD, Zabolotna DD i dr. 2013. Sravnitelnoe izuchenie soderzhaniya sekretornogo immunoglobulina A v rotoglotochnom sekrete i syivorotke krovi lyudey, provedennoe razlichnyimi metodami i reaktivami. ImunologIya ta alergologiya. 2: 88-91.

8. Suvorovtsev VI, Fedorov TV, Gusev VV. 2001. Bakterialnyie IgA1 proteazyi: poluchenie, svoystva perspektivyi primeneniya. Vestnik RAMN. 12: 39—42.

9. Tjabringa G, Vos JB, Olthuis D et al. 2005. Host defense effector molecules in mucosal secretijns. Fems Immunology & Med Microbiology. 45: 151—158. http://dx.doi.org/10.1016/j.femsim.2005.03.004; PMid:16051067