SOVREMENNAYA PEDIATRIYA.2015.7(71):115-118; doi 10.15574/SP.2015.71.115

Polymorphic variants of TGF-β1 genes in pyelonephritis affected by the vesicoureteral reflux in infants

Tokarchuk N. I., Odarchuk I. V., Zaichko N. V. 
N.I. Pirogov Vinnitsa National Medical University, Ukraine

Objective: to determine the plasma concentration of TGF-B1 in children with pyelonephritis and to study the effect of allelic polymorphisms of TGF-B1 (-509 C/T, +869 T/C) on the predisposition to the development of nephrosclerosis pyelonephritis on the background of vesicoureteral reflux in children of early age.

Materials and methods. Under observation there were 68 patients with pyelonephritis who were on the treatment course in Khmelnytsky regional children's hospital. The first group consisted of 36 children signs of vesicoureteral reflux. The second group consisted of 32 children sick with pyelonephritis on the background of the vesicoureteral reflux. The content of TGF-B1 was determined by enzyme immunoassay (ELISA). Determination of polymorphisms of TGFB1 (P-T and T+S) was performed by polymerase chain reaction in real time.

Results. Most children with pyelonephritis on the background of the vesicoureteral reflux defines atypical variants of alleles of the gene TGF-B1 (-509 CC in 90,62% and +869 TT in 81.25%), which can be a predictor of the formation of fibrosis of the renal parenchyma. Atypical variants of the gene TGF-B1 (SS -509 and +869 TT) are identified more often in boys than in girls. In children with vesicoureteral reflux II and III article showed an atypical variant (-509СС) gene of TGF-B1. But when І grade reflux was significantly more frequently identified polymorphism of TGF-B1 (+869 TT). A strong correlation between the gene polymorphism of TGF-B1 and the plasma concentration of this cytokine indicate that the overproduction of TGF-B1.

Conclusion. Promising remains the further study of pathogenetic aspects of the formation of irreversible changes in kidneys with pyelonephritis on the back-ground of vesicoureteral reflux in children of early age.

Key words: pyelonephritis, vesicoureteral reflux, infants, transforming growth factor, gene polymorphism.

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