Ukrainian Journal of Perinatology and Pediatrics. 2023. 4(96): 15-23; doi: 10.15574/PP.2023.96.15
O.V. Bulavenko, L.R. Ostapiuk, V.O. Rud, A.S. Voloshinovskii, T.S. Malyi
National Pirogov Memorial Medical University, Vinnytsia, Ukraine

For citation: Bulavenko OV, Ostapiuk LR, Rud VO, Voloshinovskii AS, Malyi TS. (2023). Modern methods of the diagnostic of pathology of pregnancy and the postpartum period. Ukrainian Journal of Perinatology and Pediatrics. 4(96): 15-23; doi: 10.15574/PP.2023.96.15.
Article received: Jul 06, 2023. Accepted for publication: Dec 15, 2023.

Postpartum purulent-inflammatory diseases are currently a serious problem.
Purpose – to develop and implement of a new modern method of the diagnosis, monitoring and assessment of health of women during pregnancy, before childbirth and in the postpartum period – the method of fluorescence spectroscopy – to ensure the provision of highly qualified medical care, including the effective treatment.
Materials and methods. The research material consisted of blood serum samples of 265 patients. There were 40 pregnant women (the Group 1), 40 women with an uncomplicated postpartum period (the Group 2), 170 – with postpartum purulent-inflammatory diseases (the Group 3), 15 patients with sepsis (the Group 4). Diagnostic methods: clinical, laboratory, instrumental, method of fluorescence spectroscopy, statistical (logistic regression and ROC analysis methods).
Results. In the Group 1 there is an increase in the intensity of fluorescence to 1.3 r.u. For the Group 2 the intensity of fluorescence was within 0.86-1.0 r.u. For the Group 3, the decrease in fluorescence intensity from 1 to 0.35 r.u. was recorded. For the Group 4 in addition to decreasing the fluorescence intensity, a noticeable shift of the fluorescence spectra of blood serum into the long-wave range was recorded.
Conclusions. A reliable prognostic model of the development of postpartum purulent-inflammatory diseases was created. The modern approach for the diagnosis and effective control of treatment within the framework of the method of fluorescent spectroscopy and biomarkers using infusions of donor albumin solutions is proposed.
The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of participating institution. The informed consent of the patient was obtained for conducting the studies.
No conflict of interest was declared by the authors.
Keywords: postpartum purulent-inflammatory diseases, the method of fluorescence spectroscopy, albumin, the pathogenetic concept, the prognostic model.

REFERENCES

1. Agnello L, Ciaccio M. (2023). Biomarkers of Sepsis. Diagnostics. 13: 435. URL: www.mdpi.com/journal/diagnostics. https://doi.org/10.3390/diagnostics13030435; PMid:36766539 PMCid:PMC9914708

2. Agnello L, Iacona A, Maestri S et al. (2021). Independent Validation of Sepsis Index for Sepsis Screening in the Emergency Department. Diagnostics. 1: 1292. https://doi.org/10.3390/diagnostics11071292; PMid:34359375 PMCid:PMC8306244

3. Agnello L, Iacona A, Sasso BL et al. (2021). A new tool for sepsis screening in the Emergency Department. Clin. Chem. Lab. Med. 59: 1600-1605. https://doi.org/10.1515/cclm-2021-0208; PMid:33851525

4. Agnello L, Sasso BL, Vidali M et al. (2021). Validation of monocyte distribution width decisional cut off for sepsis detection in the acute setting. Int. J Lab. Hematol. 43: 183-185. https://doi.org/10.1111/ijlh.13496; PMid:33634941

5. Agnello L, Vidali M, Sasso BL et al. (2022). Monocyte distribution width (MDW) as a screening tool for early detecting sepsis: A systematic review and meta-analysis. Clin. Chem. Lab. Med. 60: 786-792. https://doi.org/10.1515/cclm-2021-1331; PMid:35166088

6. Bakhtawar S, Sheikh R, Qureshi Z et al. (2020). Risk factors for postpartum sepsis: a nested case-control. BMC Pregnancy and Childbirth. 20; 297: 1-7. https://doi.org/10.1186/s12884-020-02991-z; PMid:32410594 PMCid:PMC7227107

7. Bulavenko O, Ostapiuk L, Rud V. (2021). Problems and Challenges to Women's Reproductive Health in the 21th Century. Acta Scientific Women's Health. Special Issue 3: 70-87. https://doi.org/10.31080/ASWH.2021.SI.03.0012

8. Bulavenko O, Ostapiuk L, Voloshinovskii A et al. (2020). A Prognostic Model of the Development of Postpartum Purulent-Inflammatory Diseases. International Journal of Clinical Medicine. 11: 32-42. https://doi.org/10.4236/ijcm.2020.112004

9. Bulavenko OV, Gerych ID, Ostapiuk LR et al. (2013). Modeling of Blood Serum Changes in Patients with Various Diseases and Treatment Measures. Biomedical and Biosocial Anthropology. 20: 8-14.

10. Bulavenko OV, Ostapiuk LR, Rud VO et al. (2019). Method of Early Diagnosis of Postpartum Purulent-Septic Complications Using the Method of Fluorescence Spectroscopy. Patent No. 133472 Ukraine GO1N 33/48 (2006.01) GO1N 21/64 (2006.01). Applicant and Patentee: Pirogov Vinnytsia National Medical University. No. u2018 10669; 29.10.2018; 10.04.2019, Bull. No. 7: 4.

11. De Berardis D, Mazza M, Marini S et al. (2014). Psychopathology, emotional aspects and psychological counselling in infertility: a review. Clin Ter. 165(3): 163-169.

12. Faix JD. (2013, Jan-Feb). Biomarkers of sepsis. Crit Rev Clin Lab Sci. 50 (1): 23-26. Free PMC article. Review. https://doi.org/10.3109/10408363.2013.764490; PMid:23480440 PMCid:PMC3613962

13. Finnerty CC, Herndon DN, Chinkes DL, Jeschke MG. (2007, Jan). Serum cytokine differences in severely burned children with and without sepsis. Shock. 27 (1): 4-9. https://doi.org/10.1097/01.shk.0000235138.20775.36; PMid:17172973

14. Fleischmann-Struzek C, Goldfarb DM, Schlattmann P et al. (2018). The global burden of paediatric and neonatal sepsis: a systematic review. The Lancet Respiratory medicine. 6 (3): 223-230. https://doi.org/10.1016/S2213-2600(18)30063-8; PMid:29508706

15. Gerych I, Levitska L, Voloshinovskii A. (2003). Luminescent analysis as a method of diagnosis of sepsis. Bulletin Lviv. university. Ser. biological. 32: 23-30.

16. Gerych ID, Bulavenko OV, Ostapiuk LR et al. (2012). Method for Early Diagnosis of Septic Complications by the Method of Fluorescence Spectroscopy. Patent No. 76953 Ukraine А61В 17/00 G01N 33/48, G01N 21/64. Applicant and Patentee: Pirogov Vinnytsia National Medical University. No. 201207441; stat. 19.06. 25.01.2013. Bull. No. 2: 4.

17. Gerych ID, Bulavenko OV, Ostapiuk LR. (2014). Spectral-Fluorescent Properties of Serum as a Reliable Marker for Early Diagnosis of Sepsis. Journal Gynecology and Obstetrics. 2: 71-74. https://doi.org/10.11648/j.jgo.20140205.11

18. Hung K-Y, Tsai Y-H, Lin C-Y et al. (2021). Application of Peak Glucose Range and Diabetes Status in Mortality Risk Stratification in Critically Ill Patients with Sepsis. Diagnostics. 11: 1798. https://doi.org/10.3390/diagnostics11101798; PMid:34679496 PMCid:PMC8534908

19. Jamison DT, Feachem RG, Makgoba et al. (2006). Disease and mortality in sub-Saharan Africa. World Bank Washington, DC.

20. Kovalenko O, Ostapiuk L, Voloshinovskii A. (2023). Current Problems of the Diagnostics and Treatment of Sepsis and Burn Injuries: The Modified Pathogenetic Concept. International Journal of Clinical Medicine. 14: 20-33. https://doi.org/10.4236/ijcm.2023.141002

21. Kyriakoudi A, Rovina N, Koltsida O et al. (2022). Weaning Failure in Critically Ill Patients Is Related to the Persistence of Sepsis Inflammation. Diagnostics. 12: 92. https://doi.org/10.3390/diagnostics12010092; PMid:35054259 PMCid:PMC8774440

22. Ostapiuk L. (2019). Diagnostic and Therapeutic Model of Sepsis and Purulent-Inflammatory Diseases. International Journal of Clinical Medicine. 10: 577-595. https://doi.org/10.4236/ijcm.2019.1011047

23. Rooney KL, Domar AD. (2018, Mar). The relationship between stress and infertility. Dialogues Clin Neurosci. 20 (1): 41-47. https://doi.org/10.31887/DCNS.2018.20.1/klrooney; PMid:29946210 PMCid:PMC6016043

24. Sari MI, Ilyas S. (2022). The Expression Levels and Concentrations of PD-1 and PD-L1 Proteins in Septic Patients: A Systematic Review. Diagnostics. 12; 8. https://doi.org/10.3390/diagnostics12082004; PMid:36010357 PMCid:PMC9407082

25. Terreno E, Galliano M, Fanali G et al. (2005). The extraordinary ligand binding properties of human serum albumin. IUBMB Life. 57 (12): 787-796. https://doi.org/10.1080/15216540500404093; PMid:16393781

26. WHO. (2016). Fact sheet of maternal mortality. URL: https://www.who.int/news-room/fact-sheets/detail/maternal-mortality. Accessed 10 Mar 2019.

27. World Health Organization. (2014). Trends in maternal mortality: 1990 to 2013: estimates by WHO, UNICEF, UNFPA, The World Bank and the United Nations Population Division: executive summary. Geneva.

28. Yagmur E, Abu Jhaisha S, Buendgens L et al. (2022). Clusterin Plasma Concentrations Are Decreased in Sepsis and Inversely Correlated with Established Markers of Inflammation». Diagnostics. 12: 3010. https://doi.org/10.3390/diagnostics12123010; PMid:36553017 PMCid:PMC9776480

29. Yoon J, Kym D, Hur J et al. (2023). Validation of Sepsis-3 using survival analysis and clinical evaluation of quick SOFA, SIRS, and burn-specific SIRS for sepsis in burn patients with suspected infection. PLoS ONE. 18(1): e0276597. https://doi.org/10.1371/journal.pone.0276597; PMid:36595535 PMCid:PMC9810178