SOVREMENNAYA PEDIATRIYA.2016.6(78):107-110; doi 10.15574/SP.2016.78.107 

Immunohistochemical research of wall of ureter for children with congenital megaurete

Tertyshniy S., Spahi O., Kokorkin A.

Zaporozhye State Medical University, Ukraine

Congenital megaureter is one of the most serious defects of the urinary system. The frequency of this disease remains high and population studies of the data from 10 to 20% in the overall structure malformations of the urinary tract in children in recent years.

Objective. To conduct a comparative analysis of the results of immunohistochemical study of intraoperative material infants with megaureter.

Materials and methods: observed and treated 15 children aged 3 to 12 months with congenital megaureter II–IV degree. We conduct a comparative immunohistochemical study of operational material objectification degree of reduction smooth muscle component in the wall of the ureter, depending on the severity of the neuro-muscular dysplasia. Used monoclonal antibodies to cytoplasmic actin antigen expressed on smooth muscle cells.

Results and discussion: the characteristic structure changes and statistically significant differences that characterize the poorly expressed, moderate and severe neuromuscular dysplasia recorded in the wall of the ureter in children with megaureter. In mild dysplasia area of α-SMA positive cells was 54.19% in the wall of the ureter, with moderate dysplasia — 43.43%, while heavy — 29.61%. Pathological changes in the muscular layer of the ureter are inversely proportional to dystrophic and degenerative changes of the cover transitional epithelium, which together determine the motor dysfunction of the ureters and promotes the development of secondary renal disease, dictates the need for surgical correction of the defect as soon as possible observations.

Conclusion: Our study emphasizes the importance of taking into account indicators of immunohistochemistry in predicting the outcome of surgical treatment and its timing in infants with congenital megaureter.

Keywords: immunohistochemistry, megaureter children.

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