• Evaluation of microflora of the vagina in women with bacterial vaginosis after treatment to determine its effectiveness 
en To content

Evaluation of microflora of the vagina in women with bacterial vaginosis after treatment to determine its effectiveness 

HEALTH OF WOMAN. 2016.1(107):154–159; doi 10.15574/HW.2016.107.154 
 

Evaluation of microflora of the vagina in women with bacterial vaginosis after treatment to determine its effectiveness 
 

Tovstanovska V. A., Alatorskyh A. E., Parsal Faranak

A.A. Bogomolets National Medical University, Kiev 
 

Objective: evaluation by qualitative and quantitative composition of microflora of the vagina and comparable characteristics of different treatments to determine the effectiveness of the treatment. 
 

Materials and methods. Using clinical, microscopy, qualitative and quantitative polymerase chain reaction methods for the determination of conditionally pathogenic and pathogenic flora of the urogenital tract. 
 

Results. It proved the importance of determining the composition and quantitative ratio of the vaginal microbiota in bacterial vaginosis (BV) for the selection of personalized therapy. A optimal diagnostic algorithm for optimizing the choice of BV causal treatment. 
 

Conclusion. According to the results of the survey demonstrated that patients receiving personalized therapy, taking into account the sensitivity of the identified micro-organisms associated with BV and their amounts were stable efficacy of treatment in 97.5% of cases, which indicates the visible personalized therapy advantage over the standard two-step treatment. 
 

Key words: bacterial vaginosis, microbiocenosis, the effectiveness of treatment. 
 

REFERENCES

1. Aksenova O A 2005. Modern aspects of clinic, diagnostics and treatment of B.V. in women of reproductive age:157.

2. Dobrokhotova YE, Zatikyan NG 2010. Microbiocenosis of the vagina. Aspects of hormonal regulation: textbook.-method. Allowance:20.

3. Ancyrskaya AS, Mouravieva VV 2006. Laboratory diagnosis of opportunistic infections of the vagina. Consilium medicum 7;3:206-210.

4. http://biomed-mdl.com.ua/info/doctors/femoflor.html

5. http://www.piluli.kharkov.ua

6. https: //health. mail. ru/ drug/ clindamycin_1/

7. Apolikhina IA. Muslimov SZ 2008. Bacterial vaginosis: what's new? Gynecology 10;6:36-37.

8. Isaeva AS, Letarov AV, Ilyina E N 2012. Species identification of vaginal lactobacilli isolated from women of reproductive age. Obstetrics and gynecology 3:60-64.

9. Tsvelev JV 2013. Terminology in obstetrics, gynecology and Perinatology: textbook:386.

10. Belokrysenko SS 2010. Dysbacteriosis from a microbiologist`s standpoint. Clinical laboratory diagnostics 8:47-49.

11. Briese V, Neumann G, Waldschla?ger J. 2010. Efficacy and tolerability of a local acting antiseptic agent in the treatment of vaginal dysbiosis during pregnancy. Arch Gynecol Obstet. 43;5:215–220.

12. Ravel J, Gajer P, Abdo Z. 2010. Microbes and Health Sackler Colloquium: Vaginal microbiome of reproductive-age women. Proc Natl Acad Sci U S A. 24;16:23–31.

13. Branco KM, Nardi RMD, Moreira JLS. 2010. Identification and in vitro production of Lactobacillus antagonists from women with or without bacterial vaginosis. Braz J Med Biol Res. 43;4:338–344. http://dx.doi.org/10.1590/S0100-879X2010007500013

14. Kostava MN 2008. Microbiocenosis of the vagina and the condition of the cervical epithelium. Gynecology 10;6:42-44.

15. Kafarskaia LI, Efimov B A, Artemova LV, Pokrovskaya M. S. Microecology of the vagina. Microbiocenosis in normal and pathological States and methods of its correction. http://lages-lab.ru/article_16.htm.

16. Rudnev OD, A. Dobretsova T, Makletsova SA, ed. by Radzinsky VE 2013. The recurrence of bacterial vaginosis and lactoflora: from actual ambiguity to practical solutions. Editorial Board StatusPraesens:16.

17. Atassi F., Servin A.L. 2010. Individual and co-operative roles of lactic acid and hydrogen peroxide in the killing activity of enteric strain Lactobacillus johnsonii NCC933 and vaginal strain Lactobacillus gasseri KS120.1 against enteric, uropathogenic and vaginosis-associated pathogens. FEMS Microbiol Lett. 1;304:29–38. http://dx.doi.org/10.1111/j.1574-6968.2009.01887.x; PMid:20082639

18. Yamamoto T, Zhou X, Williams CJ 2009. Bacterial Populations in the Vaginas of Healthy Adolescent Women. J. Pediatr. Adolesc. Gynecol. 22;1:11–18. http://dx.doi.org/10.1016/j.jpag.2008.01.073; PMid:19232297

19. Sudarikova E G, Belimov SI 2010. The state of vaginal microbiocenosis in patients in assisted reproductive technologies. Russian Bulletin of obstetrician-gynecologist 2:49-51.

20. Voroshilina ES, Plotko EE, Hawtin VN 2006. Laboratory criteria for prescribing antibiotics in the treatment of urogenital mycoplasmosis. Ural medical journal. Microbiology. Special issue:63-65.

21. Mavzyutov AR, Bondarenko KR, Bondarenko VM 2007. Bacterial vaginosis: etiopathogenic aspects. Journal Of Microbiology 6:93-100.

22. Coudeyras S., Jugie G., Vermerie M., Forestier C. et al. 2008. Adhesion of human probiotic Lactobacillus rhamnosus to cervical and vaginal cells and interaction with vaginosis-associated pathogens. Infect Dis Obstet Gynecol. 27:549–640.

23. Gelber S.E., Aguilar J.L., Lewis K.L.T., Ratner A.J. 2008. Functional and phylogenetic characterization of vaginolysin, the human-specific cytolysin from Gardnerella vaginalis. J. Bacteriol. 190:3896–3903. http://dx.doi.org/10.1128/JB.01965-07; PMid:18390664 PMCid:PMC2395025

24. Rampersaud R., Planet P.J., Randis T.M. et al. 2010. Inerolysin, a cholesterol-dependent cytolysin produced by Lactobacillus iners. J. Bacteriol. 193:1034–1041. http://dx.doi.org/10.1128/JB.00694-10; PMid:21169489 PMCid:PMC3067590

25. Macklaim J.M., Fernandes A.D., Bella JM.Di. 2013. Comparative meta-RNA-seq of the vaginal microbiota and differential expression by Lactobacillus Iners in health and dysbiosis. Microbiome. 1:12. http://dx.doi.org/10.1186/2049-2618-1-12; PMid:24450540 PMCid:PMC3971606

26. Prilepskaya VN, Bayramova GR, Ancyra A. S. 2009. Vaginal microecosystem vagina in norm and at a pathology. Gynecology 11;3:9-11.

27. http://pmarchive.ru/chto-nuzhno-znat-o-bakterialnom-vaginoze/

28. Tsydenova C. B. 2010. Diagnosis and treatment of bacterial vaginosis (review). Division Bulletin SB RAMS 2:248-255.

29. Kira E. F. 2012. Bacterial vaginosis. M, LLC "Medical information Agency:472.

30. Sukhih GT, Trofimov DY., Burmenskaya OV. 2011. The profile of cytokine gene mRNA expression in vaginal smears of women of reproductive age with non-specific vaginitis and bacterial vaginosis. Obstetrics and gynecology 7-2:33

31. Diaz-Cueto L, Dominguez-Lopez P, Tena-Alavez G. 2009. Effect of clindamycin treatment on vaginal inflammatory markers in pregnant women with bacterial vaginosis and a positive fetal fibronectin test Int. J Gynaecol Obstet. 107;2:143–146. http://dx.doi.org/10.1016/j.ijgo.2009.06.015; PMid:19647824

32. Bondarenko KR, Gaisina YuR, Akhmadullina YA 2011. Immunopathogenetic effects of lipopolysaccharides of gram-negative bacteria in bacterial vaginosis. Mother and child: Mater. V Regional scientific forum:171-172.