HEALTH OF WOMAN.2017.1(117):85–88; doi 10.15574/HW.2017.117.85

Ostapenko O. I., Kvashenko V. P., Akimova I. K., Nosova I. N.
LLC «Institute of family planning», Kyiv

The objective: the study of immunomodulatory effects of a probiotic, which contains lyophilized Lactobacillus (Lactobacillus rhamnosus) – 13 mg (2,0ґ109 CFU) and lyophilized bifidobacteria (Bifidobacterium lactis) – 4 mg (2,0ґ109 CFU) the level of serum immunoglobulin IgA as a marker of local immunity in the plasma of women of reproductive age with the violation of the biocenosis of the vagina.

Patients and methods. The study involved 86 patients of reproductive age with the violation of the vaginal biocenosis, which were divided into two groups according to received treatment.

A survey was conducted for all patients in both groups: determine the level of serum IgA, measuring pH of vaginal environment and the quantification of lactobacilli and pathogenic flora with the help of test-system «Florotsenoz» before treatment and in 6 weeks after treatment. The state of vaginal microbiocenosis in both groups before treatment was homogeneous. Patients in both groups as therapy at the first stage of treatment received, if necessary antimicrobial therapy depending on the selected flora. In the second stage (restoration of microflora) patient of the main group received systemic probiotic combined with a complex prebiotic local action, patients in the control group, the probiotic localy in the form of the vaginal candles or tablets.

Results. The research stated the increasing level of serum IgA in blood plasma of patients of the main group compared to control group at 20%, normalizing the pH of the vaginal environment in the main group in 94% of cases, which indicates an increase of immunity in mucosal.

Conclusion. The inclusion of the systemic probiotic in the scheme of treatment of disorders of biocenosis of the vagina system enhances the increasing of immunity of the mucous membranes, and the vaginal tablets prebiotic of local action restores the own normal microflora of the vagina.

Key words: serum immunoglobulin A, local immunity, vaginal dysbiosis, probiotics, prebiotics, vaginal microbiocenosis, the pH of the vaginal environment.

REFERENCES

1. Besedin VM, Zhemela OM. 2012. Klinichne zastosuvannya shvidkih testiv dlya viznachennya rN vaginalnih vidilen. Medichni aspekti zdorov’ya zhinki4 (56).

2. Bondarenko VM, Rubakova EI, Lavrova VA. 1998. Immunostimuliruyuschee deystvie laktobakteriy, ispolzuemyih v kachestve osnovyi preparatov probiotikov. Zhurn. mikrobiol., epidemiol., immunobiol. 5:107–112.

3. Volodin NN, Korshunov VM, Gladko IA, Kafarskaya LI, Sidorov AA, Ahmetova LM. 1986. Vliyanie mikrofloryi rodovyih putey beremennyih na techenie perioda novorozhdennosti nedonoshennyih detey. Voprosyi ohranyi materinstva i detstva 31;3:53–56.

4. Radzinskiy VE, Knyazev SA, Kostin IN. 2009. Akusherskiy risk: maksimum informatsii – minimum opasnosti dlya materi i mladentsa. M, Eksmo.

5. Fukushima Y, Kawata Y, Hara H, Terada A, Mitsuoka T. 1998. Effect of a probiotic formula on intestinal immunoglobulin A production in healthy children. Int J Food MicrobioI 42:39–44. https://doi.org/10.1016/S0168-1605(98)00056-7

6. Cebra JJ. 1999. Influences of microbiota on intestinal immune system development. Am J Clin Nutr 69:1046S–1051S. PMid:10232647

7. Link-Amster H, Rochat F, Saudan KY, Mignot О, Aeschlimann JM. 1994. Modulation of a specific humoral immune response and changes in intestinal flora mediated through fermented milk intake. FEMS Immunol Med MicrobioI 10:55–63. https://doi.org/10.1111/j.1574-695X.1994.tb00011.x

8. Marteau P, Vaerman JP, Dehennin JP, Bord S, Brassart D, Pochart P et al. 1997. Effects of intrajejunal perfusion and chronic ingestion of Lactobacillus johnsonii strain Lal on serum concentrations and jejunal secretions of immunoglobulins and serum proteins in healthy humans. Gastroenterol Clin BioI 21:293–8.

9. Spanhaak S, Havenaar R, Schaafsma G. 1998. The effect of consumption of milk fermented by Lactobacillus casei strain Shirota on the intestinal microflora and immune parameters in humans. Eur J Clin Nutr 52:899–907. https://doi.org/10.1038/sj.ejcn.1600663; PMid:9881885

10. Quan CP, Berneman A, Pires R, Avrameas S, Bouvet JP. 1997. Natural poly­ reactive secretory immunoglobulin. A autoantibodies as a possible barrier to infection in humans. Infect Immun 65:3997–4004. PMid:9316998 PMCid:PMC175574

11. Rumyantseva T., Bellen G., Savochkina Y., Guschin A., Donders G. Diagnosis of aerobic vaginitis by quantitative real-time PCR..Received: 11 October 2015. Accepted: 29 December 2015 Springer-Verlag Berlin Heidelberg 2016.

12. Wheeler JG, Bogle ML, Shema SJ, Shirrell MA, Stine KC, Pittler AJ et al. 1997. Impact of dietary yogurt on immune function. Am J Med Sci 313:120–3. Med MicrobioI 1994;10:55–63. https://doi.org/10.1016/S0002-9629(15)40069-2; https://doi.org/10.1097/00000441-199702000-00011; PMid:9030681