SOVREMENNAYA PEDIATRIYA.2016.6(78):80-83; doi 10.15574/SP.2016.78.80 

Cytological study of tonsils functional state in children with type 1 diabetes and chronic tonsillitis

Gavrylenko Iu., Layko A., Volgina I.

Shupyk National Medical Academy of Postgraduate Education, Kiev, Ukraine

National Children Specialized Hospital «Ohmatdet», Kiev, Ukraine

The purpose of present work — the study of the functional state of the tonsils (T) in children, patients with DM-1 and CT based on the study of cytologic features of the crypts contents (T).

Materials and Methods. To address this goal 51 children were examined, aged 6 to 18 with type-1 diabetes mellitus and CT —14 girls (27.5%), boys — 37 (72.5%), average age 11,5±4,6. The duration of the DM-1 was from 1 to 10 years. All the children, except for clinical and laboratory examination, according to the рrotocol, have passed through cytologic study of crypts content (T). Cytological investigations of Pappenheim stained smears were performed by microscopy (x100, x1000).

Results. The studies in children, patients with DM-1 showed some cytological features that characterize different functional state of T in the presence of CT. Compensated functional capacity T is characterizied by moderate or high degree of squamous epithelium desquamation; polymorphonuclear leukocytes in moderate amounts, sometimes macrophages; lymphocytes, partly in the state of blast transformation, in a small or moderate amounts, sometimes plasma cells, separately and in clusters; histiocytes in a small amount, eosinophils — occasionally. Mixed bacterial flora in moderate or large amount. Decompensation of functions of T is characterized by moderate and high degree of squamous epithelium desquamation with attributes of cell proliferation or degenerative changes. In smears one can find polynuclear giant cells of chronic inflammation. Neutrophil leukocytes with unfinished phagocytosis of bacteria in large amount, sometimes macrophages, histiocytes, sometimes single lymphocytes or missing. Rich mixed bacterial flora, including spirochetes, fusobakteria, sometimes fungi.

Conclusion. Thus, cytology of T crypts content in children, patients with DM-1 and CT can serve as an additional informative examination method of the functional state of T assessment, CT form specification and evaluation of its conservative treatment effectiveness.

Keywords: chronic tonsillitis, cytology, diabetes mellitus type 1, children

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