PERINATOLOGIYA I PEDIATRIYA.2014.4(60):14–17;doi10.15574/PP.2014.60.14

Confined placental mosaicism: mechanisms of origin and the prospects for prediction

Gordiyenko I.Yu. ,  Nikitchina T.V.,  Vashchenko O.A., Tarapurova E.N. , Velichko A.V.,  Bolyukh V.M.

SI «Institute of Pediatrics, Obstetrics and Gynecology, NAMS of Ukraine», Kiev, Ukraine

Object — to conduct the analysis of the results of cytogenetic examinations of chorion and placenta in high_risk pregnant women.

Patients and methods. A total of 3724 high-risk pregnant women were under observation. Prenatal ultrasound examinations were performed in real time with the use of ACCUVIX V20EX-EXP, ACCUVIX V10LV-EX, Aloka SSD — 630, HDI 4000 devices. Invasive procedures were conducted under the standard, according to the testimony. For cytogenetic studies of chronic biopsy and the placenta was used the direct method of fixing [Flori E. et al., 1985; Baranov V.S. et al., 1990] in the own modification. Culturing and fixing of cord blood lymphocytes was conducted by the use of semimicromethod [Hungerford D. et al., 1965]. Preparations of chromosomes were analyzed by the use of light microscopy BX51 (Olympus) and BX53 (Olympus) extended at x10000. The analysis results are recorded on the international nomenclature (ISCN, 2013).

Results. Confined placental mosaicism was detected in 23 (0.62%) cases. By virtue of additional research the normal karyotype of the fetus was diagnosed in 19 (0.5%) cases, and chromosomal pathology — only in 4 (0.1%). The false negative result is obtained in 2 cases (in fetuses of different sex).

Conclusions. All cases of the confined placental mosaicism requires detailed further examination.

Key words: confined placental mosaicism, chromosomal abnormality, invasive prenatal diagnosis.

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