SOVREMENNAYA PEDIATRIYA.2017.4(84):96-100; doi 10.15574/SP.2017.84.96

Chernyshova L. I., Teslenko M. Yu., Radionova N. M., Kasyan O. I.
Shupyk National Medical Academy of Postgraduate Education, Kyiv, Ukraine
Kyiv Municipal Children's Clinical Hospital No. 1, Ukraine

Objective. To determine the burden of rotavirus infection and to characterize the severity of clinical manifestations in children younger than 5 years.

Materials and methods. The study was conducted by standard protocol approved by the World Health Organization. Determining the severity of the disease was performed by using point scale assessing the severity of clinical manifestations of rotavirus gastroenteritis Vesikari.

Results and discussion. The study involved 218 children, rotavirus etiology confirmed in 92 (42.2%) cases. Genotyping was conducted in 34 samples of biological material. Children younger than 2 years of age accounted for 61.92% (57/92). Children were admitted to the hospital on the 1th — 7th day of illness

(Median 2 [IQR: 1–3]). At the time of admission, 72.83% (67/92) of them had vomiting and 82.67% (76/92) of children had fever. During the height of disease, the diarrhea was predominant on the frequency and duration. The clinical course of rotavirus gastroenteritis according to the Vesikari Score in most children was estimated as severe (14 points (2.86)). During 2015 in Kyiv were circulating simultaneously 8 genotypes, and the most common was Genotype G4P8

that made up to 50% (17/34). The latter caused severe clinical course according to the Vesikari Score.

Conclusions. Rotavirus infection characterized by severe clinical course in children younger than 5 years, and therefore, a large number of hospitalized patients. The significant burden of rotavirus infection in children of this age group proves the feasibility of universal vaccination.

Key words: rotavirus, Vesikari scale, genotypes, vaccination.

Referenses

1. Chernyshova LI, Teslenko MY, Radionova NM et al. (2016). The long-term monitor rotavirus in children under 5 years are hospitalized in 2 centers Ukraine 2006-2015. The Health of child. 7(75): 117-123.

2. Mihailova EV. (2006). Rotavirus infection in children. А textbook. Saratov: 80.

3. Kharchenko YuP, Yurchenko IV, Eremenko SA, Kashintsev SV, Pitel AA. (2014). Modeling the severity and variants of the course of rotavirus diarrhea in children. Actual Infectology. 2(3): 24-26.

4. Rotavirus vaccine. The document from a position of WHO – January 2013 Weekly Epidemiological Bulletin. 5: 49-64. http://www.who.int/wer

5. Saluja T, Dhingra MS, Sharma SD et al. (2016, Sep 29). Association of rotavirus strains and severity of Gastroenteritis in Indian children. Hum Vaccin Immunother. doi 10.1080/21645515.2016.1238994].

6. Mwenda JM, Ntoto KM, Abebe A et al. (2010). Burgen of epidemiology of rotavirus diarrhea in selected African countries: preliminary result from the African Rotavirus Surveilance Network. J Infect Dis. 202; suppl: 5-11. https://doi.org/10.1086/653557; PMid:20684718

7. Kawai K, O’Brien MA, Goveia MG, et al. (2012). Burgen of rotavirus gastroenteritis and distribution of rotavirus strains in Asia: a systematic review. Vaccine. 30: 1244-1254. https://doi.org/10.1016/j.vaccine.2011.12.092; PMid:22212128

8.Bahl R, Ray P, Subodh S et al. (2005). Delhi Rotavirus Stady Group. Incidence of severe rotavirus diarrhea in New Delhi, India, fnd G and P types of the infection rotavirus strains. J Infect Dis. 192; suppl 1: 114- 119. https://doi.org/10.1086/431497; PMid:16088794

9. Svraka S, Duizer E, Vennema H et al. (2007). Etiological role of viruses in outbreaks of acute gastroenteritis in The Netherlands from 1994 through 2005. Journal of clinical microbiology. 45(5): 1389-1394. https://doi.org/10.1128/JCM.02305-06.

10. Chhabra P, Payne DC, Szilagyi PG et al. (20130. Etiology of viral gastroenteritis in children <5 years of age in the United States, 2008–2009. The Journal of infectious diseases. 208(5): 790-800. doi 10.1093/inf dis/jit 254.

11. Subijanto Marto Sudarmo, Katsumi Shigemura, Alpha Fardah Athiyyah, Kayo Osawa. (2015). Genotypihg and clinical factors in pediatric diarrhea caused by rotaviruses: one-year surveillance in Surabaya, Indonesia. Gut Pathogens. 7: 3. https://doi.org/10.1186/s13099-015-0048-2

12. Ghana Statistical Servises. Ghana Health Service and ICF Macro. Ghana Demographic and Health Survey 2008. Accra. Gnana

13. Podkolzin AT, Fenske EB, Abramycheva NYu. (2009). Hospital-Based Surveillance of Rotavirus and other Viral Agents of Diarrhea in Children and Adults in Russia, 2005–2007. The Journal of Infectious Diseases. 200: 228–233. https://doi.org/10.1086/605054; PMid:19817602

14. Gentsch JR, Glass RI, Woods P. (1992). Identification of group A rotavirus gene 4 types by polymerase chain reaction. J Clin Microbiol. 30: 1365-73.  PMid:1320625 PMCid:PMC265294

15. Kapikian AZ, Chanock RM. (1996). Rotaviruses. Fields Virology 3rd ed. Philadelphia, PA: Lippincott-Raven: 1659.

16. Manual of rotavirus detection and characterization methods (WHO/IVB/08.17). (2013). World Health Organization. Geneva. 2009. http://www.who.int/nuvi/rotavirus/WHO_IVB_08.17_eng.pdf.

17. Parashar UD, Bresee JS, Gentsch JR, Glass RI. (1998). Rotavirus Emerg Infect Dis 4(4): 561-570. https://doi.org/10.3201/eid0404.980406.

18. Hu L, Crawford SE, Hyser JM, Estes MK, Venkataram Prasad BV. (2012). Rotavirus non-structural proteins: structure and function. Current Opinion in Virology. 2: 380–388. https://doi.org/10.1016/j.coviro.2012.06.003; PMid:22789743 PMCid:PMC3422752

19. Linhares AC, Verstraeten T, Wolleswinkel van der Bosch J, Clemens R, Breuer T. (2006). Rotavirus serotype G9 is associated with more-severe disease in Latin America. Clin Infect Dis. 43: 312-4. https://doi.org/10.1086/505493; PMid:16804845

20. Ruuska T, Vesikari T. (1990). Rotavirus disease in Finnish children: use of numerical scores for clinical severity of diarrhoeal episodes. Scand J Infect Dis. 22: 259-267. https://doi.org/10.3109/00365549009027046.