Perinatology and pediatric.Ukraine.2017.4(72):99-105; doi 10.15574/PP.2017.72.99

Antypkin Y. G., Chumachenko N. G.
SI «Institute of Paediatrics, Obstetrics and Gynecology of NAMS of Ukraine», Kyiv, Ukraine

Objective: to develop additional non-invasive criteria for diagnosis of bronchial asthma in children from an ecologically unfavourable region on the basis of exploration of the amino acid composition of the serum blood and the exhaled breath condensate.
Material and methods. The material for research was the serum of blood and the condensate of expired air, collected in children aged 6-17 years old. In the sampling material the following amino acid composition was determined: lysin, histidin, arginine, asparaginic acid, threonine, serine, glutamic acid, glycine, alanine, cysteine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine and ammonia. In total 48 children from Dneprodzerzhinsk were examined, 28 of them with bronchial asthma and 20 apparently healthy children. The children with asthma were divided into groups depending on the onset of acute asthmatic attack, history of allergies and the severity of the disease. During the examination, all patients were in the stage of clinical remission.
Results. The associations of positive family history for allergic reactions in children with bronchial asthma and the serum concentration of glycine and methionine were proved. The detected changes of amino acid composition in the serum and exhaled breath condensate indicate the considerable oxidative stress and specificity of amino acid exchange that is typical for the children with bronchial asthma from an ecologically unfavourable region.
Conclusions. Changes in the spectrum of amino acids in the blood serum (glycine and methionine) in children with a positive history of allergies can be an additional prognostic diagnostic criterion for asthma.
Key words: amino acids, bronchial asthma, children, exhaled breath condensate, blood serum.

References

1. Antipkin YuG, Radchenko N, Nadtochiy T. (2010) Studying of amino acids in a condensate of exhaled air at children with nonspecific diseases of organs respiratory apparatus. Perinatologiya i pediatriya. 4(44): 56-60.

2. Antipkіn YuG, Lapshin VF, Umanets’ TR. (2010). Clinical guidelines on diagnostics and treatment of a bronchial asthma at children. Health of Ukraine. 3(14): 39–41.

3. Burtseva SA, Postolache OM, Bratukhina AA. (2012). Еffect of electromagnetic radiation millimeter range on protein and amino acid composition of biomass streptomyces. Electronic treatment of materials. 48(4): 76–82.

4. Vorotniak TM. (2009). Improvement of treatment of the children with bronchial asthma according to activity of the chronic bronchial inflammation. Extended abstract of diss … candidate med. sciences. Odesa: 31.

5. Anokhina TN, Anaev EK, Revelsky AI et al. (2011). Diagnostic value of semi-volatile organic compounds in exhaled breath condensate of asthma and chronic obstructive pulmonary disease patients. Pulmonology.(4): 71-75. 

6. Donos AA, Garayeva SN, Leorda AI. (2015). Рrofile free amino acids in blood serum as the immune status of sickly children and children with acute pneumonia. Eurasian Union of Scientists (ESU). 12(21): 60-63.

7. Zavalskaya TV. (2015). Blood plasma essential aminoacids of stable and unstable stenocardia patients. NAS of Ukraine. 5: 173-179.

8. Anaev EKh, Kushaeva ME, Kurova VS. (2012). A role of proteomic analysis of exhaled breath condensate in diagnosis of chronic obstructive pulmonary disease and pneumonia. Pulmonology. 5: 5–9.

9. Kazak SS, Vіlensky AB, Soldatov OV. (2007). A replaceable amino acid is an irreplaceable constituent of holiatry of chronic diseases for children and teenagers. Child’s Health. 6(9): 21-28.

10. Klimenko VA, Kryvorotko DN. (2011). Analysis of air expelled How marker biochemically processes in the body. Child’s Health. 1(28): 138-143.

11. Kopylov PhYu, Syrkin AL, Chomakhidze PSh. (2013). Рathology diagnostics by human breath analyze. Klinicheskaya meditsina. 10: 16-21.

12. Lytvynets LYa.( 2013). Oxidative stress and antioxidant defense  in children with varying degrees of bronchial asthma control. Child’s Health. 8(51): 71-74.

13. Gryn’ NV, Burlaka YuB, Shuklina YuV et al.(2013). Оn diagnostic value of content and distribution of free amion acods in blood of patients with inflammatory and oncologic diseases of upper respiratory tract. Laboratory diagnosis. 2 (64): 14-18.

14. Polonikov AV, Ivanov VP, Bogomazov AD et al. (2015). Genetic and biochemical mechanisms of involvement of antioxid ant defense enzymes in the development of bronchial. Biomedical Chemistry. 4; 61: 427-439.

15. Kononikhin AS, Fedorchenko KYu, Ryabokon AM et al. (2015). Рroteomic anal ysis of exhaled brea th condensa te for diagnosis of pa thologies of the respira tory system. Biomedical Chemistry. 61; 6: 777–780.

16. Fedorchenko KU, Ryabokon AM, Kononikhin AS et al. (2016). Lung cancer early diagnosis based on proteome analysis of exhaled breath condensate. Bulletin of Moscow Universit . Series 2 : Chemistry: 112–120.

17. Rusyn VI, Sirchak YeS, Kurchak NYu. (2013). Тhe content of free amino acids in blood serum of patients with chronic pancreatitis. Gastroenterology. 3(49): 123-126.

18. Severin ES. (2003). Biochemistry: a textbook for high schools. M.: GEOTAR – MED. 779.

19. Soodaeva SК. (2012). Free radical mechanisms of injury in respiratory Disease. Pulmonologiya. 1: 5-10.

20. Ryabokon AM, Anaev EKh, Kononikhin AS. (2014). Comparative proteomic analysis of exhaled breath condensate in patients with lung carcinoma using high resolution mass-spectrometry. Pulmonologiya. 1: 5–11. 

21. Fyodorov VI, Karapuzikov AA, Starikova MK. (2013). Рroteins, peptides and amino acids as markers of bronchopulmonary diseases. 12; 6: 167–174.

22. Fedoseeva NM, Perelman JM. (2008). Тhe role of free radical oxydation inpathogenesisof bronchial asthma and airway hyperresponsiveness. A Вulletin Producing. 29: 38-44.

23. Sheybak VM, Pavlyukovets AYu. (2013). Аnd ymmunnaya arginine system vozmozhnыe mechanisms interaction. Journal VSMU. 12; 1: 6-13.

24. Shmulich OV. (2014). Аmino acid blood pool of children with allergic diseasesAnnals of Mechnikov Institute. 1: 57-60.

25. Borrill ZL, Roy K, Singh D. (2008). Exhaled breath condensate biomarkers in COPD. Eur Respir J. 32(2): 472—486.

26. Syslova K, Kacer P, Kuzma M et al. (2012). Determination of Biomarkers in Exhaled Breath Condensate: A Perspective Way in Bronchial Asthma Diagnostics. Bronchial Asthma — Emerging Therapeutic Strategies. Ed. E. Sapey. InTech Rijeka Croatia: 37—74.

27. Fischer BM, Pavlisko E, Voynow JA. (2011). Pathogenic triad in COPD: oxidative stress, protease anti-protease imbalance, and inflammation. Int J Chron Obstruct Pulm Dis. 6: 413—421. https://doi.org/10.2147/COPD.S10770; PMid:21857781 PMCid:PMC3157944

28. Global strategy for asthma management and prevention. National institutes of health. National Heart, lung and Blood Institute. Revised 2015. http://www.ginasthma.org.

29. Papadopoulos NG et al. (2012). International consensus on (ICON) pediatric asthma. Allergy. —— 67; 8: 976—997.

30. MacNee W. (2009). Accelerated lung aging: a novel pathogenic mechanism of chronic obstructive pulmonary disease (COPD). Biochem Soc Trans. 37; Pt 4: 819—823.

31. Montuschi P. (2007). Analysis of exhaled breath condensate in respiratory medicine: methodological aspects and potential clinical applications. Therapeutic Advances in Respiratory Disease. 1(1): 5—23.

32. Umit M Sahiner, Esra Birben, Serpil Erzurum (2011, Oct). Oxidative Stress in Asthma. World Allergy Organ J. 4(10): 151—158.

33. Conventz A, Musiol A, Brodowsky C et al. (2007). Simultaneous determination of 3-nitrotyrosine, tyrosine, hydroxyproline and proline in exhaled breath condensate by hydrophilic interaction liquid chromatography/electrospray ionization tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 860; 1: 78-85.