SOVREMENNAYA PEDIATRIYA.2016.4(76):120-123; doi10.15574/SP.2016.76.120 

Features bacteriological spectrum infected and purulent wounds in children 

Spakhi O. V., Paholchuk A. P.

Zaporozhye State Medical University, Ukraine 

Wound infection occupies an important place among the causes of surgical morbidity among children. Evidence of this is the tendency to increase the number of patients with purulent-inflammatory diseases of soft tissues in the last 20 years. To develop methods for antibiotic treatment of inflammatory infections is important etiological microbiological monitoring structure.

The aim of our study was to investigate changes in the quantitative and qualitative composition of pathogens of wound infection when the infected and festering wounds in children.

Materials and methods. The results of bacteriological studies in 220 patients with purulent and infected wounds of soft tissues. Age of children was from 3 months to 17 years. The criterion of the etiological role of pathogens was titles CFU / ml. Isolation and culture of microorganisms was carried out according to standard methods in accordance with modern algorithms microbiological studies. Determination of the species was carried out on bacteriological analyzer VITEK 2 Compact.

Results and its discussion. The analysis bacteriological crop discharge from the wounds showed that the microflora of purulent wounds mainly sown gram-positive cocci, which is 94.6%±2.5% isolates. Among them priority pathogen was Staphylococcus aureus, 54.4% is allocated to the crops.

Conclusions. The microflora of the infected and purulent wounds in children is dominated by gram-positive cocci, including staphylococci predominate in 79.2% of cases. Bacteriological spectrum infected and purulent wounds in children represented 81.7% monoculture. Prospects for further study of the problem may be related to the investigation of possible local use of antibiotics in the treatment of infected and purulent wounds.

Key words: infected and purulent wounds, microorganisms, children.

REFERENCES

1. Blatun LA, VA Mitin, Terekhova RP. 2014. Eplannovy preparation for topical treatment of skin and soft tissue infections in a multidisciplinary hospital. Journal of them. Prof.B.M. Kostyuchenko wounds and wound infections. 1: 6—12.

2. Winnick S, Markelov NM, Tyuryumin VS. 2013. Modern methods of treatment of purulent wounds. Siberian medical review. 1: 18—24.

3. Golubov A, Privolnaya VV. 2014.Local antibiotic treatment of surgical soft tissue infections in the outpatient setting. Journal of them. Prof.B.M. Kostyuchenko wounds and wound infections. 1: 33—37.

4. Shamaeva SH, Mironov A.Yu., Petrov KM, Sveshnikov NN. 2012. Microbiological monitoring of pathogens in surgical wounds of patients and their susceptibility to antimicrobial agents. Kursk scientific-practical herald "Man and his health". 1: 138—141.

5. Shkvarkovsky IV, Antoniuk TV, Iftodi AG Bilyk AV, Crushed AB. 2013. The use of vacuum cavitation rehabilitation methods in treatment of necrotic processes. Georgian Medical News. 12(225): 16—21.

6. Adaev AM, Bobrovnikova My, Dyachenko SV, Kutsiy MB. 2013. Wound infections. Pharmacoepidemiological and bacteriological monitoring. Journal of Public Health and Health Russian Far East. 2: 56—59.

7. Khvorostov ED, Morozov SA, Zakharchenko Yu, Tomin MS. 2009. Ultrasonic cavitation of purulent wounds. Kharkiv surgical school. 2.1(33): 64—66.

8. Shpynev KV, Krechikova OI, Krechikov VA, Kozlov RS. 2007. Streptococcus pyogenes: characterization of the microorganism, separation, identification and determination of sensitivity to antibiotics. Guidelines // Clinical Microbiology and Antimicrobial Chemotherapy. 9; 2: 104—120.

9. Fluit AC, Verhoef J, Schmitz FJ. 2001. Frequency of isolation and antimicrobial resistance of gramnegative and grampositive bacteria from patients in intensive care unit of 25 European university hospitals participating in the European arm of the SENTRY antimicrobial surveillance Program 1997—1998. European Journal of Clinical Microbiology and Infections Diseases. 20: 617—625. http://dx.doi.org/10.1007/s10096-001-8078-8; PMid:11714042

10. Rosenthal VD et al. 2008. International Nosocomial Infection Control Consortium report, data summary for 2002—2007. Am J Infect Control Nov. 36(9): 627—637. http://dx.doi.org/10.1016/j.ajic.2008.03.003; PMid:18834725

11. Klenerman L. 2007. A history of osteomyelitis from the Journal of Bone and Joint Surgery: 1948 to 2006. J Bone Joint Surg Br. 89; 5: 667—670. http://dx.doi.org/10.1302/0301-620X.89B5.19170; PMid:17540756

12. Washington C Winn Jr. et al. 2006. Koneman's Color Atlas and Textbook of Diagnostic Microbiology. 6th ed. Lippincott Williams & Wilkins: 1535.

13. Leclercq R. 2009. Epidemiological and resistance issues in multidrug-resistant staphylococci and enterococci. Clinical Microbiology and Infection. 15; 3: 224—231. http://dx.doi.org/10.1111/j.1469-0691.2009.02739.x; PMid:19335370

14. National Committee for Clinical Laboratory Standards. Performance standards for antimicrobial susceptibility testing; tenth informational supplement. NCCLS Document. 2012. 32; 3: 23—24.

15. Pulgar S, Mehra M, Quintana A et al. 2008. The epidemiology of hospitalized cases of skin and soft tissue infection in Europe. 18th European Congress of Clinical Microbiology and Infectious Diseases. Barcelona, Spain, abstr. P. 821.