PERINATOLOGIYA I PEDIATRIYA. 2014. 3(59):51-54; doi 10.15574/PP.2014.59.51 
 

Dynamics levels of cytokines allergic inflammation in the use of vitamin D in children with allergic diseases

А.V. Tyazhka, Z.V. Selska

Bogomolets national medical university, Kyiv, Ukraine

Purpose. Determination of IL-4 and IL-10 levels in children with allergic diseases during use of vitamin D in their different doses and at different periods of the disease.

Materials and methods. We observed 16 children with allergic diseases. Determination of 25(OH)D in serum and IL-4, IL-10 levels in children were initially after application of vitamin D at a dose of 2000 IU daily for two months, and after years of pores after application of vitamin D in the high dose — 4000 IU daily during remission and 5000 IU daily during the acute illness, course of treatment for 2 months. 11 patients each definition 25-gidroksikaltsiferolu cytokines and allergic inflammation in the blood was carried out in the period of exacerbation of the disease, and 5 patients — in remission of the disease. Definition 25(OH)D was performed using the method elektrochemiluminestsencysi Eleksys 2010 analyzer (Roche Diaggnostics, Germany) test system Cobas. Determination of IL-4 and IL-10 in blood was performed using kits of reagents for immunoassay.

Results. A significant difference between the levels of IL-4 in children with allergic diseases in acute illness and after the application of the primary dose cholecalciferol 2000 IU daily for 2 months (Z=2.845; p<0.05), also found a significant difference between the performance of children initially and after the summer (Z=2.934; p<0.05) and the primary and after applying 4.000 IU (Z=2.934; p<0.05). It was established that the rate of IL-10 was significantly lower than after ingestion of vitamin D 4000–5000 IU compared with after summer (Z=2.023; p<0.05), and after receiving a dose of Vitamin D 2.000 IU (Z=2.023; p<0.05). All children with allergic diseases observed relief of the underlying disease and the improvement of the general condition after the use of vitamin D in the complex therapy of allergic diseases. Significant therapeutic effect was observed after administration of high doses of cholecalciferol (4000–5000 ME daily for 2 months).

Conclusions. Thus, our findings have shown that vitamin D is involved in the allergic inflammation. With this in mind it is advisable to assume that cholecalciferol has appointed in remission and exacerbation of the disease and presumably dose of vitamin D is to increase in the period of exacerbation of the disease, since the need for this vitamin in exacerbation of the disease increases.

Key words: vitamin D, treatment of allergic inflammation cytokines, remission of the disease, worsening of the disease.

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