• Features of Helicobacter Pylori infection course in children depending on tool-like receptor-4 genotypes
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Features of Helicobacter Pylori infection course in children depending on tool-like receptor-4 genotypes

PERINATOLOGIYA I PEDIATRIYA.2013.4(56):66–70

 

Features of Helicobacter Pylori infection course in children depending on tool-like receptor-4 genotypes

 

Gerasimenko O. N.

State Institution «Dnipropetrovsk Medical Academy of the Ministry of Health of Ukraine », Dnepropetrovsk, Ukraine

 

 

Objective. To study the expression patterns of TLR4 in gastric mucosal biopsy and nuclear factor-κB in lymphocytes, the concentration of sCD14 in the blood serum depending on H. Pylori-status and SNP Asp299Gly TLR4 gene of children with chronic gastroduodenal pathology (CGDP).

 

 

Patients and methods. The study was conducted among 66 children (in the age 7–17 years) with CGDP in the acute stage. Depending on the presence of H. pylori patients were divided into two groups: I (main) group — patients with positive H. Pylori-status (44 or 66.7%), II (comparison) group – children without H. pylorі (22, 33.3%). A complex clinical, laboratory and instrumental examination, endoscopic examination of the esophagus, stomach and duodenum by the standard technique (FEGDS), (Pentax FG-15W, Japan), ultrasound examination of the abdominal cavity is conducted. H. Pylori-status identified with the use of «Helpil» rapid urease test and «Helic» breathing test (LLC «AMA», Russia, St. Petersburg).

 

 

Results. The interaction of molecular components TLR4 of associated excitation depending on the status of H. Pylori-state and TLR4 genotype is shown. Association between the intensity of clinical and endoscopic manifestations and level of specific immune response to virulence factor SagA Ag H. pylori in children with Helicobacter pylori infection is found.

 

 

Conclusions. Inflammation in the gastric mucosa (GM) in children with polymorphism Asp299Gly TLR4 gene is highly sensitive to the protective effect sCD 14. Availability Asp299Gly TLR4 gene polymorphism in children is accompanied by a high level of influence on the intensity of the activity of TLR4 specific immune antibody response to infection with H. pylori. Among children with Helicobacter pylori infection without TLR4 gene polymorphism observed synchrony excitation TLR4 expression in GM and release mechanisms sCD14, which balance underlies the primary regulation of TLR4 dependent activity of the inflammatory process .

 

Key words: Helicobacter pylori, innate immunity, TLR4, sCD14, NF-κB, SNP Asp299Gly gene TLR4, children.

 

 

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