HEALTH OF WOMAN. 2016.8(114):75–77; doi 10.15574/HW.2016.114.75 
 

Identification and characterization of antibodies against human Hsp60 at pregnancy 
 

Makarenko M., Govsieiev D., Vorona R., Tsisarenko A., Vasil’tchuk O., Pavlyuk O., Yakovenko L., Kroupska I., Pogribna A., Sidorik L.

Institute of postgraduate education, National medical University named after O. O. Bogomolets, Kyiv

Institute of molecular biology and genetics, NAS of Ukraine, Kyiv

Heat shock proteins (HSPs) or molecular chaperones were originally described as members of the cell response to different types of stress (infection, the influence of heavy metals, ischemia, hypoxia, amino acid starvation, psycho-emotional stress, hormonal stress, etc.). Hsp60 is one of the first proteins that are synthesized during embryogenesis. Hsp60 is necessary for the development of the embryo.

The objective: was the identification and characterization of anti-Hsp60 autoantibodies in pregnant women to assess the prospects for a possible use as additional capabilities for the prediction of pregnancy progress.

Patients and methods. Was a group of 170 pregnant women. 20 pregnant women were clinically healthy (control group) and 150 pregnant women had complicated anamnesis (main group). The level of IgG anti-Hsp60 antibodies in the serum was determined using immunoassay (ІFA). Immunoreactivity was determined by Western blot (immunoblotting).

Results. Using the ІFA method, anti-Hsp60 positive serum was detected in 7.7% of pregnant women from the control group and in 14.0% of pregnant with complicated anamnesis. A statistically significant difference between the level of anti-Hsp60 autoantibody (on average) in pregnant women of the main group and the control group was not identified (0.177±0.118 vs. 0.134±0.097 units of optical density, p>0.05). Also, it was determined the frequency of detection of pathologies in pregnant women which have an anti-Hsp60 positive serum, compared with pregnant women which have an anti-Hsp60 negative serum.

Conclusions. Our results suggest that in relation to the anti-Hsp60 antibody studies as an additional test, in particular, the forecast of progress of pregnancy, joint use ІFA and Western blotting is more appropriate.

Key words: pregnancy, Hsp60, anti-Hsp60 autoantibodies.

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