• The study prevalence of polymorphisms of genes of detoxification system in children with inflammatory bowel disease 
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The study prevalence of polymorphisms of genes of detoxification system in children with inflammatory bowel disease 

PERINATOLOGIYA I PEDIATRIYA. 2016.3(67):118-122; doi 10.15574/PP.2016.67.118 
 

The study prevalence of polymorphisms of genes of detoxification system in children with inflammatory bowel disease 
 

Berezenko V. S., Tkalik E. N., Rossokha Z. I., Dyba M. B.

SI «Institute of Pediatrics, Obstetrics and Gynecology of NAMS of Ukraine», Kyiv, Ukraine

SI «Reference-centre for molecular diagnostic Ministry of Public Health of Ukraine», Kyiv, Ukraine


Purpose — investigation of the detoxification genes (GSTM1, GSTT1, MDR1) polymorphism distribution in population of children with inflammatory bowel disease and evaluation of its potential clinical utility in comparison with the current publications in the field.


Patients and methods. The study involved 45 children with inflammatory bowel diseases in the age from 3 to 18 years old. Clinical study was conducted under the order of Ministry of Health of Ukraine (№ 59 from 29.01.2013). Deletions polymorphism detection of GSTM1 and GSTT1 genes was performed by multiplex polymerase chain reaction, while polymorphic variants of gene MDR1 (C3435T) were detected by polymerase chain reaction, followed by analysis of restriction fragment length polymorphism.


Results. GSTM1 gene deletion was detected in 27 children with inflammatory bowel disease (60%), while in Ukrainian population this value is a bit lower (54.4%, χ2=0.45; p=0.5). The frequency of GSTM1 gene deletions in patients with Crohn's disease and ulcerative colitis was observed at 71.5% and 54.8% respectively (χ2=1.1; p=0.34). GSTT1 gene deletion was detected in 26.7% of children with inflammatory bowel disease, while frequency of this mutation in Ukrainian population is 20.1% (χ2=0.9; p=0.34). Study of MDR1 gene polymorphism revealed TT-genotype at 22.3%, while in Ukrainian population this variant reaches 26.8% (χ2=0.45; p=0.50). ST-genotype was found in 53.4% of children with inflammatory bowel disease, while in Ukrainian population it is 59.0% (χ2=0.1; p=0.74).


Conclusions. No significant difference observed between distribution of the genetic variants of the genes GSTM1, GSTT1, MDR1 in population of children with inflammatory bowel diseases and in the control group. Mutations of the noted genes cannot be considered to be the risk factors for development of inflammatory bowel diseases in children.


Key words: children, inflammatory bowel disease, ulcerative colitis, Crohn's disease, detoxification genes.


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