• The genes expression which is encoding enzyme key reactions of folate-dependent metabolism in human placenta in the first and third trimesters of uncomplicated pregnancy
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The genes expression which is encoding enzyme key reactions of folate-dependent metabolism in human placenta in the first and third trimesters of uncomplicated pregnancy

PERINATOLOGIYA I PEDIATRIYA.2014.4(60):24–30;doi10.15574/PP.2014.60.24


The genes expression which is encoding enzyme key reactions of folate-dependent metabolism in human placenta in the first and third trimesters of uncomplicated pregnancy

K.L. Korneyeva1, R.R. Rodriges1, S.V. Ralchenko2, A.V. Vakulenko2, L.V. Manzhula3, V.T. Melnik4, O.Yu. Vereshchak4, M.Yu. Obolenskaya1


1Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kiev, Ukraine

2Taras Shevchenko Natsionalny University, Kiev, Ukraine

3Maternity Hospital № 3, Kyiv, Ukraine

4Irpin Maternity Hospital, Irpin, Ukraine



Introduction. Folate-dependent metabolism of one-carbon fragments – is a metabolic system of interconnected reactions responsible for basic biological processes in the cell: development and division, regulation of gene expression by means of methylation. According to the recent studies was found the course of pregnancy and the health of the mother and fetus due to the operation of this system.


Purpose — to determine the gene expression of this system responsible for the synthesis of nucleic acid precursors, and accordingly for the proliferation, the expression of genes responsible for the synthesis of the intracellular energy carriers, as well as for the synthesis of methionine and the dependent methylation processes; provide a quantitative characterization of the expression of presented genes at the level of RNA in the placentas of the first and the third trimesters of pregnancy.


Method. Polymerase chain reaction method was used in the work.


Results. In comparison with the early stages of pregnancy, in the mature placenta was found three_time reduction of RNA containing, encoding the last stage of the purines and methionine synthesis. The reactions of the previous folate-dependent stages of the purine synthesis and cytidine monophashate methylation during the process of thymidylate synthesis hardly changed at the end of pregnancy in comparison with the first trimester.


Conclusions. These results can be as a baseline quantitative data for evaluation of changes in the system of folate-dependent metabolism of one-carbon fragments in pathological conditions and during the all period of pregnancy.


Key words: folate-dependent metabolism of one-carbon fragments, gene expression, uncomplicated pregnancy.






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