• Rationale for using probiotic, containing strains of Lactobacillus reuteri DSM 17938 and ATCC PTA 5289, in children with chronic generalized catarrhal gingivitis

Rationale for using probiotic, containing strains of Lactobacillus reuteri DSM 17938 and ATCC PTA 5289, in children with chronic generalized catarrhal gingivitis

SOVREMENNAYA PEDIATRIYA.2018.3(91):119-125; doi 10.15574/SP.2018.91.119

Trubka I. A., Savichuk N. O., Tsyhanko I. R.
Shupyk National Medical Academy of Postgraduate Education, Kyiv, Ukraine

Objective: to investigate the influence of the probiotic that contains Lactobacillus reuteri DSM 17938 and Lactobacillus reuteri АТСС РТА 5289 on the detection rate of periodontopathogens and biochemical indicators of oral fluid in school-aged children with chronic generalized catarrhal gingivitis (CGCG).

Material and methods. Clinical trial included 84 children aged 7–15 years with mild-to-moderate CGCG, who were divided into two groups according to age: group I — 7–11 years, group II — 12–15 years. Depending on the use of the probiotic preparation, there were two subgroups: the control treatment group (CTG) and main treatment group (MTG); the latter included children who were administered probiotic containing strains of Lactobacillus reuteri DSM 17938 and Lactobacillus reuteri АТСС РТА 5289. The oral fluid was assayed for such biochemical parametres and electrolytes as Са, Р, acid and alkaline phosphatase, MDA, catalase. The molecular genetic study was based on the genomic DNA extraction from the major periodontopathogenic bacteria in crevicular fluid: Prevotella intermedia, Bacteroides forsythus, Treponema dendicola, Actinobacillus actinomycetem comitans, Porphyromonas gingivalis.

Results. A significant detection rates of periodontopathogens in MTG was established, which caused the increased levels of acid and alkaline phosphatase, MDA and decreased level of catalase. Providing 2 courses of treatment with the topical probiotic preparation BioGaia ProDentis in both age groups significantly reduced the detection rates of periodontopathogens in MTG and, as a result, improved the biochemical markers and electrolytes in the oral fluid. Clinical findings indicate that carrying out two treatment courses using topical probiotic preparation with Lactobacillus reuteri DSM 17938 and Lactobacillus reuteri ATCC PTA 5289 significantly reduced the detection rates of periodontopathogens in children of MTG that, as a result, improved the biochemical parameters of their oral fluid. Clinically, we observed the decrease of hygienical and dental plaque indices, amelioration of clinical signs of chronic catarrhal gingivitis, namely, signs of inflammation and gingival bleeding, which resulting in prolonged remission.

Conclusions. The obtained positive results of clinical study confirm the necessity of inclusion of the topical probiotic preparation BioGaia ProDentis in the complex of therapeutic and preventive measures in school9aged children with chronic catarrhal gingivitis.

Key words: probiotics, Lactobacillus reuteri, periodontopathogens, children, chronic generalized catarrhal gingivitis.

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