• Protozoynye invasion of urogenital system in combine with bacterial vaginosis agents by women of fertil age and their sexual partners
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Protozoynye invasion of urogenital system in combine with bacterial vaginosis agents by women of fertil age and their sexual partners

HEALTH OF WOMAN. 2018.7(133):100–106; doi 10.15574/HW.2018.133.100

Fedorich P. V.
Ukrainian Military Medical Academy, Kyiv

Study of protozoyny invazions of the uroganital system with combination of microorganisms which are associated with bacterial vaginosis by women of fertil age and their sexual partners men is perspective direction of modern medicine. The development of this direction can increase the level of diagnostic inspection of patients by transmissible mainly sexual way infections and improve of efficiency of their treatment.

The objective: exposure of combine defeats of uroganital system with different kinds of trichomonas or lamblias and microorganisms which are associated with bacterial vaginosis, for the women of fertil age and their sexual partners – men. All patients have infections, transmissible mainly sexual way with a chronic process and their treatment.

Materials and methods. The special research of biomaterial, taken from the urogenital system of women of fertil age and their sexual partners-men, patients infections, transmissible mainly sexual way with a chronic process is conducted. 198 patients were inspected in clinic. Used the method of PCR, amplifier of DT-96, reagents of «Femoflor-16», praymeris for the indication of Trichomonas vaginalis, Trichomonas tenax, Pentatrichomonas hominis and Giardia lamblia.

Results. Protozoyny invasions of the urogenital system determined at 88 (44.4%) of patients. At 77 (38.8%) from them determined the differet kinds of trichomonadies: Trichomonas tenax – at 41 (20.7%), Pentatrichomonas hominis – at 28 (14.1%), Trichomonas vaginalis – at 8 (4%). Giardia lamblia determined from 11 patients (5.6%). For 92% inspected patients with a protozoal invasions were determined the increassng of indexes of microflora, associated with bacterial vaginosis.

The treatment of combine pathology of urogenital system which are caused different kinds trichomonades and microorganisms, which associated with BV, were conducted with etiotropic therapy. Seknidox used 2 tablets one time per day during 5 days. Orcipol used 1 tablets 3 or 2 times per a day (depending on weight) during 10 days.

In research took part 56 patients with chronic ITSW. By 54 patients (96.4%) were eliminated of trichomonas. The normalization microflora’s level is attained for a 31 (57.4%) patient.

Decrease of mocroflora’s, which associated with BV is marked by 24 patients (42.6%).

Conclusions. This original method of treatment, is effective, has satisfactory bearableness and can be recommended for empiric therapy of combined pathology of urogenital system, caused of protozoyny invasions (different kinds of trichomonas) and microorganisms, associated with bacterial vaginosis, for the women of fertil age and their sexual partners with chronic infections, transmissible mainly sexual a way. This agents have a certain role in development of diseases of the urogenital system.

Key words: Trichomonas vaginalis, Trichomonas tenax, Pentatrichomonas hominis, Giardia lamblia, invasion, fertil age.

REFERENCES

1. Arnoldi EK. (1999). Hronicheskiy prostatit. Rostov n/D, «Feniks»:320.

2. Bochkarev EG, Sergeev YuV, Kopyiglov VM, Ryumin DV. (2000). Aktualnyie voprosyi diagnostiki urogenitalnogo trihomoniaza. Immunopatologiya, allergologiya, infektologiya 4:77–87.

3. Bochkarev EG, Sergeev YuV. (2003). Mesto PTsR v diagnostike infektsiy, peredayuschihsya polovyim putem. Immunopatologiya, allergologiya, infektologiya 3:112–116.

4. Byikov AS, Vorobev AA, Zverev VV i dr. (2008). Atlas po meditsinskoy mikrobiologii, virusologii i immunologii – 2-e izd. M, OOO «Meditsinskoe informatsionnoe agentstvo»:340.

5. Vikipediia Elektronnyi resurs. Rezhym dostupu: https://uk.wikipedia.org/wiki/Parazytarna_invaziia.

6. Gorchakov DA. (2014). Patogeneticheskie osobennosti urogenitalnogo trihomoniaza v gendernom aspekte. Dis. kand. med. nauk. Saratov:134.

7. Guschin AE, Ryizhih PG, Berezina LA, Kulyashova LB, Mahlay NS. (2010). Molekulyarno-geneticheskoe issledovanie klinicheskogo materiala s ispolzovaniem praymerov k razlichnyim uchastkam genoma Trichomonas Vaginalis i razlichnyim vidam tsarstva Protozoa. Molekulyarnaya diagnostika. Tom III. Razdel 14. Infektsii organov reproduktsii. M:204–207.

8. Daschuk AM, Petrov BR. (2009). Venericheskie bolezni (Infektsionnyie bolezni, peredayuschiesya polovyim putem): rukovodstvo. H, S.A.M:294.

9. Kaminskiy VV, Sahanova AA, Zelenskaya MV. (2007). Sovremennyie podhodyi k terapii bakterialnyih vaginozov. K:30.

10. Kaminskiy VV, Odinokoz TA, Sumenko VV. (2007). Sovremennyiy vzglyad na problemu lecheniya bakterialnogo vaginoza. Mistetstvo lіkuvannya 7:28–29.

11. Kontseptsiia zahalnoderzhavnoi tsilovoi prohramy rozvytku systemy spetsializovanoi medychnoi dopomohy patsiientam iz zakhvoriuvanniamy shkiry ta infektsiiamy, shcho peredaiutsia statevym shliakhom, na period do 2020 roku. (2016). Ukrainskyi zhurnal dermatolohii, venerolohii, kosmetolohii 1(60):7–13.

12. Kutsevlyak VF, Lahtin YuV. (2006). Harakteristika soputstvuyuschey mikrofloryi u bolnyih parodontitom s invaziey rotovyih trihomonad. VIsnik Sumskogo derzhavnogo unIversitetu. Sumi:2.

13. Neymark SL, Neymark OS. (2014). Diskussionnyie problemyi diagnostiki i lecheniya trihomonadnoy infektsii. AsotsIatsIya akusherIv-gInekologIv UkraYini 1–2:236–239.

14. Мавров ІІ, Бєлозоров ОП, Тацька ЛС. (2000). Уніфікація лабораторних методів дослідження в діагностиці захворювань, що передаються статевим шляхом. Х, Факт:120.

14. Mavrov II, Bielozorov OP, Tatska LS. (2000). Unifikatsiia laboratornykh metodiv doslidzhennia v diahnostytsi zakhvoriuvan, shcho peredaiutsia statevym shliakhom. Kh, Fakt:120.

16. Pat.107910 Ukraina, MPK S12/Q 1/68 (2006.01), S12/Q 1/04 (2006.01), S12/N 15/11 (2006.01). Sposib vyznachennia prysutnosti Trichomonas tenax u doslidzhuvanomu zrazku ta nabir praimeriv dlia yoho zdiisnennia. Fedorych PV, Zelenyi SB zaiavnyky ta patentovlasnyky. a201407161; zaiavl. 25.06.2014; opubl. 25.02.15, Biul. № 4.

17. Pat.110759 Ukraina, MPK S12/Q 1/68 (2006.01), S12/Q 1/04 (2006.01), S12/N 15/11 (2006.01), S12/R 1/90 (2006.01). Sposib vyznachennia prysutnosti Pentatrichomonas hominis u doslidzhuvanomu zrazku ta nabir praimeriv dlia yoho zdiisnennia / Fedorych PV, Zelenyi SB zaiavnyky ta patentovlasnyky.  a201501255; zaiavl. 16.02.2015; opubl. 10.02.16, Biul. №3.

18. Pat.110767 Ukraina, MPK S12/Q 1/68 (2006.01), S12/Q 1/04 (2006.01), S12/N 15/11 (2006.01), S12/R 1/90 (2006.01). Sposib vyznachennia prysutnosti Giardia lamblia u doslidzhuvanomu zrazku ta nabir praimeriv dlia yoho zdiisnennia. Fedorych P.V., Zelenyi S.B. zaiavnyky ta patentovlasnyky.  a201505750; zaiavl. 11.06.2015 ; opubl. 10.02.16, Biul. №3.

19. Razdolskaya NV. (2009). Diagnosticheskoe znachenie tsitomorfologicheskih, kulturalnyih i immunogennyih svoystv Trichomonas vaginalis. Avtoref. diss. FGOU VPO «Voenno-meditsinskaya akademiya im. S.M. Kirova» Ministerstva oboronyi RF. Sankt-Peterburg.

20. Savicheva AM i dr. (2011). Laboratornaya diagnostika urogenitalnogo trihomoniaza: Metodicheskie rekomendatsii. SPb, N L:36.

21. Turkevych OIu, Syzon OO. (2009). Kompleksno-dyferentsiinyi pidkhid do diahnostyky ZPSSh u khvorykh z deiakymy retsydyvuiuchymy urohenitalnymy infektsiiamy (trykhomoniaz, khlamidioz, bakterialnyi vahinoz). Ukr. zhurn. dermatolohii, venerolohii, kosmetolohii 1:90–93.

22. Fedorych PV, Zelenyi SB, Sadovska OA, Dudikova KV. (2017). Porivniannia efektyvnosti diahnostyky trykhomoniazu za kulturalnym metodom ta metodom polimeraznoi lantsiuhovoi reaktsii z vykorystanniam praimeriv dlia vyiavlennia Trichomonas vaginalis, Trichomonas tenax ta Pentatrichomonas hominis. Ukrainskyi zhurnal dermatolohii, venerolohii, kosmetolohii. K. 1(64):65–69.

23. Fedorych PV, Prymak AV, Konovalova TS. (2013). Bakterialnyi vahinoz: suchasnyi pohliad na problemu. Ratsionalna terapiia ta reabilitatsiini zakhody shchodo sposobu zhyttia patsiientok. Ukrainskyi zhurnal dermatolohii, venerolohii, kosmetolohii 3(50):86–94.

24. Fedorych PV. (2012). Obhruntuvannia ta aprobatsiia oryhinalnoho sposobu vziattia biolohichnoho materialu z metoiu adaptatsii diahnostykuma Femoflor-16 dlia kilkisnoi detektsii anaerobnoi ta mikroaerofilnoi mikroflory sechostatevoi systemy cholovikiv. Ukrainskyi naukovo-medychnyi molodizhnyi zhurnal 2:155–158.

25. Fedorych PV, Slobodianiuk OM, Bazyl TS, Broide YuV, Polshkova YuV. (2010). FEMOFLOR-16 – test-systema dlia etiolohichnoi diahnostyky bakterialnoho vahinoza. Tezy dopovidei Naukovoi konferentsii molodykh vchenykh Ukrainskoi viiskovo-medychnoi akademii, m. Kyiv, 23–24 kvitnia: 40–41.

26. Fedorych PV, Prymak AV, Konovalova TS. (2013). Bakterialnyi vahinoz: suchasnyi pohliad na problemu. Ratsionalna terapiia ta reabilitatsiini zakhody shchodo sposobu zhyttia patsiientok. Ukrainskyi zhurnal dermatolohii, venerolohii, kosmetolohii 3(50):86–94.

27. Adu-Sarkodie Y, Opoku BK, Crucitti T et al. (2007). Lack of evidence for the involvement of rectal and oral trichomonads in the aetiology of vaginal trichomoniasis in Ghana. Sex Transm Infect 83:130–132. https://doi.org/10.1136/sti.2006.020941; PMid:16790560 PMCid:PMC2598609

28. Centers of Disease Control and Prevention. Sexually transmitted diseases treatment guidelines. (2015). MMWR. 64; 3: 72–75.

29. Crucitti T, Abdellati S, Ross DA et al. (2004). Detection of Pentatrichomonas hominis DNA in biological specimens by PCR. Lett Appl Microbiol 38:510–516. https://doi.org/10.1111/j.1472-765X.2004.01528.x; PMid:15130148

30. Di Benedetto MA, Di Piazza F, Amodio E, Taormina S, Romano N, Firenze A. (2012). Prevalence of sexually transmitted infections and enteric protozoa among homosexual men in western Sicily (south Italy). J Prev Med Hyg. 53:181–185. PMid:23469584

31. Eschenbach DA, Davick PR, Williams BL, Klebanoff SJ, Young-Smith K аnd Critchlow CM et al. (1989). Prevalence of hydrogen peroxide-producing Lactobacillus species in normal women and women with bacterial vaginosis. J Clin Microbiol. 27:251–256.

32. Karnaky J. (1937). JAMA. 108:24. https://doi.org/10.1001/jama.1937.02780010026006

33. Hart GJ, Elford J. (2010). Sexual risk behaviour of men who have sex with men: emerging patterns and new challenges. Curr Opin Infect Dis 23(1):39-44. https://doi.org/10.1097/QCO.0b013e328334feb1.

34. Ojonoma OL. (2008). A review of sexually transmitted diseases (STDs) of parasitic origin: The case of giardiasis. African Journal of Biotechnology 7(25):4979–4981.