• Polymorphic variants of TGF-β1 genes in pyelonephritis affected by the vesicoureteral reflux in infants
To content

Polymorphic variants of TGF-β1 genes in pyelonephritis affected by the vesicoureteral reflux in infants

SOVREMENNAYA PEDIATRIYA.2015.7(71):115-118; doi 10.15574/SP.2015.71.115

Polymorphic variants of TGF-β1 genes in pyelonephritis affected by the vesicoureteral reflux in infants

Tokarchuk N. I., Odarchuk I. V., Zaichko N. V. 
N.I. Pirogov Vinnitsa National Medical University, Ukraine

Objective: to determine the plasma concentration of TGF-B1 in children with pyelonephritis and to study the effect of allelic polymorphisms of TGF-B1 (-509 C/T, +869 T/C) on the predisposition to the development of nephrosclerosis pyelonephritis on the background of vesicoureteral reflux in children of early age.

Materials and methods. Under observation there were 68 patients with pyelonephritis who were on the treatment course in Khmelnytsky regional children's hospital. The first group consisted of 36 children signs of vesicoureteral reflux. The second group consisted of 32 children sick with pyelonephritis on the background of the vesicoureteral reflux. The content of TGF-B1 was determined by enzyme immunoassay (ELISA). Determination of polymorphisms of TGFB1 (P-T and T+S) was performed by polymerase chain reaction in real time.

Results. Most children with pyelonephritis on the background of the vesicoureteral reflux defines atypical variants of alleles of the gene TGF-B1 (-509 CC in 90,62% and +869 TT in 81.25%), which can be a predictor of the formation of fibrosis of the renal parenchyma. Atypical variants of the gene TGF-B1 (SS -509 and +869 TT) are identified more often in boys than in girls. In children with vesicoureteral reflux II and III article showed an atypical variant (-509СС) gene of TGF-B1. But when І grade reflux was significantly more frequently identified polymorphism of TGF-B1 (+869 TT). A strong correlation between the gene polymorphism of TGF-B1 and the plasma concentration of this cytokine indicate that the overproduction of TGF-B1.

Conclusion. Promising remains the further study of pathogenetic aspects of the formation of irreversible changes in kidneys with pyelonephritis on the back-ground of vesicoureteral reflux in children of early age.

Key words: pyelonephritis, vesicoureteral reflux, infants, transforming growth factor, gene polymorphism.

REFERENCES

1. Буднік ТМ. 2014. Мікробно-запальні захворювання органів сечової системи у дітей та підлітків. Методичні рекомендації. ДЗ «Луганський державний медичний університет». Луганськ: 37.

2. Морозов ДА, Моррисон ВВ, Морозова ОЛ, Лакомова ДЮ. 2011. Патогенетические основы и современные возможности ранней диагностики нефросклероза у детей с пузырно-мочеточниковым рефлюксом. Саратовский научн-мед журн. 1: 151—157.

3. Триндюк ЮС. 2011. Деякі аспекти діагностики пієлонефритів у дітей. Здоровье ребенка. 5(32): 36—39.

4. Alireza Merrikhi, Emad Bahraminia. 2014. Association of urinary transforming growth factor-β1 with the ureteropelvic junction obstruction. Adv Biomed Res. 3: 123. http://dx.doi.org/10.4103/2277-9175.133196; PMid:24949294 PMCid:PMC4063104

5. Almontaser Hussein et al. 2010. Functional polymorphismin transforming grow factor-beta-1(TGF B-1) and vascular endothelial grow factor (VEGF) genes modify risk of renal parenchymal scarring following childhood urinary tract infection. Nephrol Dial Transplant. 25: 779—785. http://dx.doi.org/10.1093/ndt/gfp532; PMid:19861314

6. Sanaa M, Abdel Salam, Safaa HA. Saleh, Eman E. El Shahawy et al. 2011. Assessment of plasma and urinary transforming growth factor beta 1 (TGF-β1) in children with lupus nephritis. Egypt J Pediatr Allergy Immunol. 9(1): 21—27.

7. Hyung Eun Yim, In Sun Bae, Kee Hwan Yoo et al. 2007. Genetic Control of VEGF and TGF-β1 Gene Polymorphisms in Childhood Urinary Tract Infection and Vesicoureteral Reflux. Pediatric Research. 62: 183—187. http://dx.doi.org/10.1203/PDR.0b013e31809871f1; PMid:17597658

8. Khalil MS, El Nahas AM, Blakemore AIF. 2012. Transforming Growth Factor-β1 SNPs: Genetic and Phenotypic Correlations in Progressive Kidney Insufficiency. Nephron Exp Nephrol. 101: 31—41. http://dx.doi.org/10.1159/000086227; PMid:15942255

9. Maimun S. 2014. The promoter region (G-800A and C-509T) polymorphisms of transforming growth factor-β1 gene among young women with recurrent urinary tract infection. Egyptian Journal of Medical Human Genetics. 15; Issue 2: 125—130.

10. Sager C, Lopez JC, Duran V et al. 2009. Transforming growth factor-beta1 in congenital ureteropelvic junction obstruction: Diagnosis and follow-up. Int. Braz J Urol. 35: 315—23. http://dx.doi.org/10.1590/S1677-55382009000300008; PMid:19538767

11. Goumenos DS, Sotiris Tsakas, Abdel Meguid El Nahas et al. 2010. Transforming growth factor-β1 in the kidney and urine of patients with glomerular disease and proteinuria. Nephrol Dial Transplant. 17(12): 2145—2152. http://dx.doi.org/10.1093/ndt/17.12.2145