- Influence of Resistol® Drug on Humoral and Cellular Immunity of Children with Recurrent and Acute Viral Respiratory Diseases: Research Findings
Influence of Resistol® Drug on Humoral and Cellular Immunity of Children with Recurrent and Acute Viral Respiratory Diseases: Research Findings
SOVREMENNAYA PEDIATRIYA.2018.8(96):104-112; doi 10.15574/SP.2018.96.104
L.V. Kvashnina, V.P. Chernyshov, I.M. Matvienko, T.B. Ignatova, D.V. Osypchuk
State Institution «Institute of Pediatrics, Obstetrics and Gynecology named after Academician O.M. Lukyanova, National Academy of Medical Sciences of Ukraine», Kyiv
NK-lymphocytes (natural killers) are the main innate immunity effectors in overcoming viral infections: they participate in the immune defense, directly eliminate virus-infected and malignantly transformed cells, activate other cells of the immune system through the secretion of cytokines. Stimulation of TLRs, and TLR3 in particular, which are expressed in almost all NK-lymphocytes, activates them, leading to secretion of cytokines, IFN-γ, and increased cellular lysis of virus-infected and tumor cells. That is, recognition of viruses through TLR3 helps NK cells play a crucial role in the first line of organism protection against viral infections. The results of the research on the influence of the medicinal product «Resistol®» on the immune defense activity in children with ARVI and recurrent upper respiratory tract infections demonstrated that on the background of «Resistol®» use the number of NK cells that could directly recognize viral pathogens increased (p<0.05). At the same time, in the group of children with acute respiratory viral infections, who did not use Rezistol®, there was no significant change in the percentage of activated NK cells in peripheral blood. In the group of children with acute respiratory infections who used Resistol®, significantly higher absolute values of leukocytes and lymphocytes were registered as compared with the group of children in whom Resistol® was not used (p <0.05), whereas at the beginning of treatment and observation these data did not differ statistically between the groups (p> 0.05).
Key words: children, humoral immunity, cellular immunity, acute respiratory viral infection, recurrent infections, Pelargonium Sidoides, Resistol®.
1. Kvashnina LV, Matvienko IN, Rodionov VP, Makovskaya YuA, Ignatova TB. (2015). Primenenie lekarstvennogo sredstva Rezistol u detey s ostryimi i hronicheskimi infektsiyami dyihatelnyih putey i nosoglotki. Pediatriya. Vostochnaya Evropa. 3(11): 2—15.
2. Chernyshova LI, Volokha AP. (2013). Dytiacha imunolohiia. Kyiv: Medytsyna.
3. Bellomo R, Bagshaw SM. (2006). Evidence-based medicine: classifying the evidence from clinical trials — the need to consider other dimensions. Crit Care. 10(5): 232. https://doi.org/10.1186/cc5045; PMid:17029653 PMCid:PMC1751050
4. Calvo M, Grob K, Bertoglio J et al. (1990). Secretory IgA deficiency in pediatric patients: clinical and laboratory follow-up. Allergol Immunopathol (Madr.). 18; 3: 149—153.
5. CD69 molecule (Homo sapiens (human)). (2006). https://www.ncbi.nlm.nih.gov/gene/969.
6. Chew V, Tow C, Huang C et al. (2012). Toll-Like Receptor 3 Expressing Tumor Parenchyma and Infiltrating Natural Killer Cells in Hepatocellular Carcinoma Patients. J Natl Cancer Inst. 104: 1796—1807. https://doi.org/10.1093/jnci/djs436; PMid:23197495 PMCid:PMC3814220
7. Conrad A, Hansmann C, Engels I et al. (2007, March 5). Extract of Pelargonium sidoides (EPs® 7630) improves phagocytosis, oxidative burst, and intracellular killing of human peripheral blood phagocytes in vitro. Phytomedicine. 14; 1: 46—51. https://doi.org/10.1016/j.phymed.2006.11.016; PMid:17184983
8. Conrad A, Jung I, Tioua D et al. (2007). Extract of Pelargonium Sidoides (EPs 7630) inhibits the interactions of group A-streptococci and host epithelia in vitro. Phytomedecine. 14; VI: 52—59. https://doi.org/10.1016/j.phymed.2006.11.018; PMid:17182236
9. Gonzales C, Williams C, Calderon V. et al. (2012). Antibacterial Role for Natural Killer Cells in Host Defense to Bacillus anthracis. Infect Immun. 80: 234—242. https://doi.org/10.1128/IAI.05439-11; PMid:22006566 PMCid:PMC3255656
10. Goulopoulou S, McCarthy C, Webb R. (2016). TollSlike Receptors in the Vascular System: Sensing the Dangers Within. Pharmacol Rev. 68: 142—67. https://doi.org/10.1124/pr.114.010090; PMid:26721702 PMCid:PMC4709508
11. Guillerey C, Chow M, Miles K et al. (2015). TollSlike receptor 3 regulates NK cell responses to cytokines and controls experimental metastasis. Oncoimmunology. 4: e1027468. https://doi.org/10.1080/2162402X.2015.1027468; PMid:26405596 PMCid:PMC4570135
12. Guo Q, Zhang C. (2012). Critical role of TollSlike receptor signaling in NK cell activation. Chin. Sci. Bull. 57: 3192. https://doi.org/10.1007/s11434-012-5257-1
13. Kayser O, Kolodziej H, Kiderlen AF. (2001). Immunomodulatory principles of Pelargonium Sidoides. Phytother Res. 15: 122—126. https://doi.org/10.1002/ptr.785; PMid:11268110
14. Koch E, Biber A. 2007 Treatment of rats with Pelargonium Sidoides extract EPs 7630 has no effect on blood coagulation parameters or on the pharmacokinetics of warfarin. Phytomedicine. 14; VI: 40—45. https://doi.org/10.1016/j.phymed.2006.11.026; PMid:17188479
15. Kolodziej H, Kayser O, Radtke OA, Kiderlen AF. (2003) Pharmacological profile of extracts of Pelargonium Sidoides and their constituents. Phytomedecine. 10; VI: 18—24. https://doi.org/10.1078/1433-187X-00307
16. Kolodziej H, Kiderlen AF. (2007). In vitro evaluation of antibacterial and immunoflamatory activities of Pelargonium reniforme, Pelargonium Sidoides and the related herbal drug preparation EPs 7630. Phytomedicine. 14; VI: 18—26. https://doi.org/10.1016/j.phymed.2006.11.020; PMid:17188480
17. Kolodziej H, Schulz V. (2003). EPs 7630: From traditional application to modern phytodrug. Deutsche Apotheker Zeitung. 143: 55—64.
18. Lizogub VG, Riley DS, Heger M. (2007). Efficacy of a Pelargonium sidoides preparation in patients with the common cold: A randomized, double-blind, placebo-controlled clinical trial. Explore. 3: 573—584. https://doi.org/10.1016/j.explore.2007.09.004; PMid:18005909
19. Moore T, Kumm P, Brown D, Petro T. (2014). Interferon Response Factor 3 is crucial to polySI:C induced NK cell activity and control of B16 melanoma growth. Cancer Lett. 346:122—128. https://doi.org/10.1016/j.canlet.2013.12.022; PMid:24368188 PMCid:PMC3963264
20. Raulet D. (2006). Missing self recognition and self tolerance of natural killer (NK) cells. Semin Immunol. 18: 145—150. https://doi.org/10.1016/j.smim.2006.03.003; PMid:16740393
21. Wines BD, Hogarth PM. (2006). IgA receptors in health and disease. Tissue Antigens. 68; 2: 103—114. https://doi.org/10.1111/j.1399-0039.2006.00613.x; PMid:16866880
22. Woof JM, Kerr MA. (2006). The function of immunoglobulin A in immunity. J. Pathol. 208; 2: 270—282. https://doi.org/10.1002/path.1877; PMid:16362985
23. Wulff S, Pries R, Wollenberg B. (2010). Cytokine release of human NK cells solely triggered with Poly I:C Cell Immunol. 263(2): 135—7. https://doi.org/10.1016/j.cellimm.2010.03.020; PMid:20427039
Article received: Aug 12, 2018. Accepted for publication: Dec 10, 2018.