• Features karyotype cell tumor clone children with different course leukemic process 
To content

Features karyotype cell tumor clone children with different course leukemic process 

SOVREMENNAYA PEDIATRIYA.2015.6(70):97-101; doi 10.15574/SP.2015.70.97 
 

Features karyotype cell tumor clone children with different course leukemic process 
 

Kucher E. V.

Shupyk National Medical Academy of Postgraduate Education, Kyiv, Ukraine 
 

Objective. To study the characteristics of the karyotype cell tumor clone in children with acute leukemia under immunophenotype of blast forms. 
 

Patients and methods. Cytogenetics (G-banding) was performed in 45 children with ALL and 28 children with AML. Molecular genetic («nested» PCR) method for studying cells in the bone marrow and peripheral blood was applied to 32 people with AML and 60 with ALL. 
 

Results. The features of the karyotype in patients with ALL and AML have been studied. The correlation between chromosomal abnormalities characteristic for different AR, and clinical features of the disease has been revealed. For AML, the characteristic genes are chimeric genes MLL-AF9, AML-ETO and CBFB-MYH11 (for M4eo variant), for ALL — chimeric genes BCR-ABLp190 and E2A/PBX1. Sophisticated failure in the karyotype of the tumor cell clone is a poor prognostic sign of the flow of leukemic children, despite the presence of specific prognostically favorable clonal disorders. 
 

Conclusions. The results indicate the feasibility of a complete cytogenetic, molecular genetic and immunological studies in children with acute leukemia for the diagnosis, differential diagnosis and prognosis of the leukemic process. 
 

Key words: acute leukemia, the karyotype, immunophenotype, forecasting, children 
 

References

1. Алексеев НА. 2009. Гематология и иммунология детского возраста. Санкт-Петербург, Гиппократ: 1040.

2. Гиндина ТЛ, Мамаев НН, Кондакова ЕВ. 2006. Цитогенетика заболеваний крови опухолевой природы. Проблемы гематологии и переливания крови. 1: 113.

3. Мамаев HH, Гиндина ТЛ, Зинина EE. 2008. Особенности течения лейкозов с повреждением локуса 1lq23. Вестник гематол. 4;3: 18—29.

4. Ребриков ДВ, Саматов ГА, Трофимов ДЮ и др. 2011. ПЦР в реальном времени. Под ред ДВ Ребрикова. 3-е изд. Москва, БИНОМ. Лаборатория знаний: 223.

5. Сергеев АГ, Иванов РА, Фечина ЛГ. 2000. Диагностическое и прогностическое значение генетических аномалий опухолевых клеток при лейкозах. Гематология и трансфузиол. 45;1: 28—35.

6. Codrington R, O'Connor HE, Jalali GR et al. 2009. Analysis of ETV6/AML1 abnormalities in acute lymphoblastic leukaemia: incidence, alternative spliced forms and minimal residual disease value. British Journal of Haematology. 3: 1071—1079.

7. Bartolo C, Viswanatha DS. 2010. Molecular diagnosis in pediatric acute leukemias. Clin Lab Med. 20: 139—182.

8. Szczepanski T, Willemse MJ, Brinkhof B et al. 2002. Comparative analysis of Ig and TCR gene rearrangements at diagnosis and at relapse of childhood precursor-B-ALL provide improved strategies for selection of stable PCR targets for monitoring of minimal residual disease. Blood. 99: 2315—2323. http://dx.doi.org/10.1182/blood.V99.7.2315; PMid:11895762

9. Jarosova M, Holzerova M, Mihal V et al. 2003. Complex karyotipes in childhood acute lymphoblastic leukemia: cytogenetic and molecular cytogenetic study of 21 cases. Cancer Gen and Cytogen. 145: 161—168. http://dx.doi.org/10.1016/S0165-4608(03)00099-2

10. Xue Y, Guo J, Wy Y et al. 2001. Cytogenetic analysis on 1058 cases of acute nonlymphocytic leukemia. 18(4): 247—250.

11. Mehrotra B, George T, Kavanau K et al. 2005. Cytogenetically aberrant cells in the stem cell compartment (CD34+lin) in acute myeloid leukemia. Blood. 86: 1139—1147.

12. Costello R, Sainty D, Lecine P et al. 2007. Detection of CBFbeta-MYH11 fusion transcripts in acute myeloid leukemia: heterogeneity of cytological and molecular characteristics. Leukemia. 11: 644—650. http://dx.doi.org/10.1038/sj.leu.2400629

13. Dohner H. 2007. Implication of the molecular characterization of acute myeloid leukemia. Hematology. 1: 412—419. http://dx.doi.org/10.1182/asheducation-2007.1.412; PMid:18024659

14. Hess JL. 2001. Detection of Chromosomal Translocation in Acute and Chronic Leukemia. American Jornal Pediatric. 87(2): 4—11.

15. Steelman IS, Pohnert SC, Shelton JG et al. 2004. JAK/STAT, Raf/MEK/ERK, PI3K/Akt and BCR-ABL in cell cycle progression and leukemogenesis. Leukemia. 18: 189—218. http://dx.doi.org/10.1038/sj.leu.2403241; PMid:14737178

16. Mrozek K, Heinonen K, Bloomfield C. 2004. Clinical importance of cytogenetics in acute myeloid leukaemia. Best Practice &Research Clinical Haematology. 14(1): 19—23. http://dx.doi.org/10.1053/beha.2000.0114; PMid:11355922

17. Roumier C, Fenaux P, Lafage M et al. 2006. New mechanisms of AML1 gene alteration in hematological malignancies. Leukemia. 17: 9—16. http://dx.doi.org/10.1038/sj.leu.2402766; PMid:12529654

18. Pui C, Gaynon PS, Boyett JM et al. 2002. Outcome of treatment in childhood acute lymphoblastic leukaemia with rearrangements of the 11q23 chromosomal region. Lancet. 359: 1909—1915. http://dx.doi.org/10.1016/S0140-6736(02)08782-2

19. Provan D, Gribben JG. 2005. Molecular hematology. Oxford, Blackwell Publishing: 324. http://dx.doi.org/10.1002/9780470987063