• Expression of VEGF, E2F8, COL6A1, IGFBP2, PLK1, RB1, RBL1 and TP53 genes in pediatric glioma
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Expression of VEGF, E2F8, COL6A1, IGFBP2, PLK1, RB1, RBL1 and TP53 genes in pediatric glioma

SOVREMENNAYA PEDIATRIYA. 2015.1(65):126-129; doi 10.15574/SP.2015.65.126


Expression of VEGF, E2F8, COL6A1, IGFBP2, PLK1, RB1, RBL1 and TP53 genes in pediatric glioma


Minchenko D. O.

Department of Pediatrics №1, National O.O. Bohomolets Medical University, Kyiv, Ukraine;

Department of Molecular Biology, Palladin Institute of Biochemistry National Academy of Sciences of Ukraine, Kyiv, Ukraine;


Objective. The goal of this study was to study in pediatric glioma the peculiarity of the expression of genes related to dysregulation of proliferation processes in malignant tumors.


Materials and Methods. The glioma tissue from 4 children with age from 5 to 8 years as well as corresponding non-malignant tissue counterparts were used in this study. RNA was isolated from glioma tissue and corresponding non-malignant tissue counterparts (control) and VEGF, E2F8, COL6A1, IGFBP2, PLK1, RB1, RBL1, and TP53 gene expressions were studied by quantitative polymerase chain reaction.


Results. It was shown that the expression level of VEGF, E2F8, COL6A1, IGFBP2, and PLK genes is increased, but RB1, RBL1, and TP-3 genes significantly decreased as compared to corresponding non-malignant tissue counterparts. More significant changes were demonstrated for COL6A1, VEGF, and IGFBP2 genes, which encoded the important regulatory proteins of extracellular matrix.


Conclusions. The obesity affects the expression of the subset of genes related to the control of glycolysis in blood cells, but insulin resistance in obesity is associated only with changes in the expression level of ENO1 and ENO2 genes, which possibly contribute to the development of insulin resistance as well as glucose intolerance.


Key words: glioma, children, gene expression, VEGF, E2F8, COL6A1, IGFBP2, PLK1, TP-3.



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