• Expression of TIMP1, TIMP2, THBS1 and THBS2 genes in blood cells of the obese adolescents with normal and impaired insulin sensitivity
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Expression of TIMP1, TIMP2, THBS1 and THBS2 genes in blood cells of the obese adolescents with normal and impaired insulin sensitivity

SOVREMENNAYA PEDIATRIYA.2015.4(68):119-122; doi 10.15574/SP.2015.68.119

Expression of TIMP1, TIMP2, THBS1 and THBS2 genes in blood cells of the obese adolescents with normal and impaired insulin sensitivity

Minchenko D. O. 
National O.O. Bohomolets Medical University, Kyiv, Ukraine
 
Palladin Institute of Biochemistry National Academy of Sciences of Ukraine, Kyiv

Objective. To study the expression of genes encoded proteins of extracellular matrix (tissue inhibitor of matrix metalloproteinases TIMP1 and TIMP2 as well as thrombospondins THBS1 and THBS2) in blood cells of obese adolescents with normal and impaired insulin sensitivity.

Materials and Methods. For this study were used three groups of adolescent boys with mean age 14 years: normal (control) and obese individuals with normal and impaired insulin sensitivity. RNA was extracted from blood cells and the levels of gene expressions were studied using quantitative real_time polymerase chain reaction.

Results: It was shown that the expression level of TIMP1 and TIMP2 genes is increased in two and four fold correspondingly, but THBS1 and THBS2 genes — decreased in the blood cells of obese adolescent boys with normal insulin sensitivity as compared to control group. Insulin resistance in obese boys leads to additional up_regulation of THBS1 and down_regulation of TIMP2, THBS1 and THBS2 genes in the blood cells as compared to obese patients with normal insulin sensitivity. Moreover, the changes in TIMP2 and THBS1 were more robust as compared to two other gene expressions.

Conclusions. It was shown the expression levels of the group of polyfunctional proteins of extracellular matrix, which control cell proliferation, are significantly deregulated in blood cells in obese adolescents with normal and suppressed insulin sensitivity and that insulin resistance in obesity is associated with changes in the expression level of all studied genes, which can contribute to the development of insulin resistance as well as glucose intolerance.

Key words: obesity, adolescents, insulin resistance, gene expressions, TIMP1, TIMP2, THBS1, THBS2, blood cells.

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