• Expression of genes, which control glucose metabolism, in the blood of the obese boys with insulin resistance
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Expression of genes, which control glucose metabolism, in the blood of the obese boys with insulin resistance

SOVREMENNAYA PEDIATRIYA.2014.6(62):112-115;doi 10.15574/SP.2014.62.112

Expression of genes, which control glucose metabolism, in the blood of the obese boys with insulin resistance

Tiazhka O. V., Minchenko D. O., Davydov V. V., Moliavko O. S., Budreiko O. A., Kulieshova D. K., Minchenko O. H. 
National O.O. Bohomolets Medical University, Kyiv, Ukraine 
Palladin Institute of Biochemistry National Academy of Sciences of Ukraine, Kyiv, Ukraine 
SI «Institute of children and adolescent health care National Academy of Medical Science of Ukraine», Kharkiv, Ukraine

Objective: To study the expression of genes, which responsible for glycolytic glucose metabolism, in the blood of obese boys with and without of insulin resistance as well as in normal (control) individuals.

Materials and Methods. The 15 boys with mean age 14 years participate in this study. They were divided into three equal groups: normal individuals as control and patients with obesity and with or without insulin resistance. Glycolytic gene expressions were studied in blood cells using quantitative polymerase chain reaction.

Results. It was shown that the expression level of aldolase C (ALDOC) and (TIGAR) genes is increased, but ENO1 and ENO2 genes — significantly does not change in the blood cells of obese boys with normal insulin sensitivity as compared to control group. Insulin resistance in obese boys leads to down-regulation of ENO1 and ENO2 genes in the blood cells as compared to obese patients with normal insulin sensitivity.

Conclusions. Results of this study provide evidence that obesity affects the expression of the subset of glucose metabolism-related genes in the blood cells and that insulin resistance in obesity is associated with changes in the expression level of ENO1 and ENO2 genes, which contribute to the development of insulin resistance as well as glucose intolerance and may reflect the changes in fat tissue.

Key words: obesity, boys, insulin resistance, mRNA expression, ALDOC, TIGAR, ENO1, blood cells.

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