- The expression of DDX58, IFIH1, IFI16, and AIM2 genes in obese adolescents and men with insulin resistance
The expression of DDX58, IFIH1, IFI16, and AIM2 genes in obese adolescents and men with insulin resistance
SOVREMENNAYA PEDIATRIYA.2017.7(87):106-111; doi 10.15574/SP.2017.87.106
Minchenko D. O.
Bohomolets National Medical University, Kyiv, Ukraine
Palladin Institute of Biochemistry National Academy of Sciences of Ukraine, Kyiv, Ukraine
Objective. To study the expression of genes encoded DDX58, IFIH1, IFI16 and AIM2 protein in lymphocytes of adolescents and adipose tissue of men in obesity associated with normal and impaired insulin sensitivity. Materials and methods. For this study adolescents and adult men were divided into three groups: normal without obesity (control) and obese individuals with normal and impaired insulin sensitivity. RNA was extracted from lymphocytes and adipose tissue and the levels of DDX58, IFIH1, IFI16, AIM2 and LEP gene expression were studied using quantitative real-time polymerase chain reaction.
Results. It was shown that the expression level of DDX58, IFIH1, IFI16 and AIM2 genes was significantly increased in lymphocytes of obese adolescents with normal and impaired insulin sensitivity as compared to control group, but insulin resistance was associated to a lesser extent with change of DDX58 gene expression and to a greater extent with change of other genes. At the same time, in adipose tissue of obese men the less noticeable changes in the induction of DDX58 and IFI16 genes were founded, but as for IFIH1 and AIM2 genes, their expression, on contrary, decreased. The level of LEP gene expression significantly increased in adipose tissue of obese men with normal and impaired insulin sensitivity. At the same time, no significant changes were found in the expression level of IFRD gene in the blood cells of obese adolescents with insulin resistance as compared to obese group with normal insulin sensitivity.
Conclusions. It was shown that the expression level of DDX58, IFIH1, IFI16 and AIM2 polyfunctional proteins, which participate in the metabolic regulation and immune response, is significantly disordered in blood cells in obese adolescents with normal and impaired insulin sensitivity and that insulin resistance in obesity is associated with significantly less changes in the expression level of DDX58 gene and to a greater extent of other genes. The observed changes in the expression of DDX58 and IFI16 genes partly reflect the changes of these gene expressions in adipose tissue.
Keywords: obesity, insulin resistance, adolescents, men, gene expressions, DDX58, IFIH1, IFI16, lymphocytes, adipose tissue.
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