• Evaluation of progressive chronic hepatitis C in children according to certain markers of oxidative stress
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Evaluation of progressive chronic hepatitis C in children according to certain markers of oxidative stress

PERINATOLOGIYA I PEDIATRIYA.2014.4(60):67–71;doi10.15574/PP.2014.60.67

Evaluation of progressive chronic hepatitis C in children according to certain markers of oxidative stress

V.S. Berezenko1, R.V. Mostovenko2, M.B. Dyba1


1The State Institution «Institute of Pediatrics, Obstetrics and Gynecology of The National Academy of Medical Sciences of Ukraine»

2The National Children's Specialized Hospital «Ohmatdyt»



Purpose — to study the peculiarities of chronic hepatitis C progression in children according to certain markers of oxidative stress.


Patients and methods. Oxidative stress was determined according to the number of ketone dinitrophenylhydrozones according to Ye.Ye. Dubinina's method. The initial level of basic 2.4 ketone dinitrophenylhydrozones (spontaneous protein oxidative modification and accumulation of basic 2.4 ketone dinitrophenylhydrozones after stimulation of protein oxidative modification by the Fenton reaction environment (induced protein oxidative modification) were determined. The rate of adaptation reserve possibilities of the organism to resist oxidative stress was calculated according to the formula (spontaneous basic 2.4 ketone dinitrophenylhydrozones x 100 / induced basic 2.4 ketone dinitrophenylhydrozones).


Results. The progression of chronic hepatitis C in children is accompanied by the increased sensitivity of serum proteins to oxidation and the accumulation of their oxidative modifications in serum. The greatest destruction of proteins under the influence of free radicals was observed in children with markers of progressive chronic hepatitis C, as well as in those suffering from liver cirrhosis. In healthy children the initiation of protein oxidative modification processes led to an average of a 30% increase of the oxidized proteins number, while in children with chronic hepatitis C the number of protein oxidative modification products was practically twice as much, and in children with liver cirrhosis a 150% increase was observed.


Conclusion. The data received testify to the depletion of adaptation reserve possibilities of the organism of children suffering from chronic hepatitis C and liver cirrhosis, as well as to their reduced ability to resist oxidative stress. A comparison of adaptation reserve possibilities rate in children with chronic hepatitis C and liver cirrhosis with ultrasound markers of fibrosis was conducted. It was determined that the adaptation reserve possibilities rate <45% can be used as a diagnostic criterion for the evaluation of progressive liver fibrosis (Sp — 76%; Se — 80%).


Key words: children, chronic hepatitis C, liver cirrhosis, protein oxidative modification, adaptation reserve possibilities, liver fibrosis.






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