• Effect of early social activity in S. pneumoniae nasopharyngeal carriage and distribution of pneumococcus serotypes in children of the first five years of life. 
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Effect of early social activity in S. pneumoniae nasopharyngeal carriage and distribution of pneumococcus serotypes in children of the first five years of life. 

SOVREMENNAYA PEDIATRIYA.2014.2(58):58-63; doi 10.15574/SP.2014.58.58 
 

Effect of early social activity in S. pneumoniae nasopharyngeal carriage and distribution of pneumococcus serotypes in children of the first five years of life. 
 

Chernysheva L. I., Gilfanova A. M., Bondarenko A. V., Yakimovitch S. A., Rabosh O. V., Yanovskaya V. V., Glushkevichi T. G., Limar T. V., Pomaz G. M., Vlasenko N. O.

P.L.Shupik National Medical Academy of Postgraduate Education, Kiev, Ukraine

Bacteriological laboratory SI «Ukrainian Center of Control and Monitoring of Diseases of the Ministry of Health Care of Ukraine», Kiev 

Objective: To study the prevalence of pneumococcal nasopharyngeal carriage and distribution of serotypes responsible for the development of invasive diseases, depending on the children's social activity and their microsocial environment. 
 

Patients and methods. A total of 743 healthy children aged from 6 months to 4 years 11 months and 30 days were examined. For the cultivation of S.pneumoniae was used bloody and «chocolate» agar. In case of negative result of bacteriological examination was conducted DNA extraction from primary material using reagent kit «DNA -sorb -AM» with the following use of PCR in the real time. 
 

Results. The overall prevalence of S.pneumoniae carriage in children is 53.8 %. Nasopharynx colonized by serotypes on 65.1 % by carriage that is most often responsible for the development of invasive pneumococcal disease. The frequency of the carriage in «home» children is 42.1%; in children attending preschools institutions — 60.4%; in orphanages — 95.6%; in large families, where multiple children attending schools the frequency of the carriage is the highest (92%) and have no difference from children's homes. 
 

Conclusions. Social activity and also presence of socially active siblings significantly effect on the prevalence of nasopharyngeal carriage of pneumococcus, especially in children up to 4 years. Use of the method of PCR helps to improve identifying of pneumococcus carriers in 2.5 times in comparison with bacteriological studies. 
 

Key words: Streptococcus pneumoniae, nasopharyngeal carriage, children, social activity. 
 

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