• Clinical significance of phospholipid levels in exhaled breath condensate in children with wheezing

Clinical significance of phospholipid levels in exhaled breath condensate in children with wheezing

SOVREMENNAYA PEDIATRIYA.2018.4(92):22-26; doi 10.15574/SP.2018.92.22

Makieieva N., Malakhova V.
Kharkiv National Medical University, Ukraine

Wheezing is a combination of clinical symptoms with a violation of the passage of air through the bronchi. The first episode of wheezing is recorded in about 30% of children in the first year. The formation of respiratory allergies most often takes its beginning in early childhood. All over the world, they relate to almost 700 million cases and about 330 million of them are bronchial asthma.
Objective. The objective of this study was to assess the levels of phospholipids in exhaled breath condensate (EBC) in children with recurrentepisode of wheezing.
Material and Methods. The objective of this study was to assess the levels of phospholipids in exhaled breath condensate in children.70 patients from 1 to 7 years withrecurrent episode of wheezing and bronchial asthma. The levels of phospholipids in EBC were studied by chromatography, using a spectrophotometer SF-46. The levels of phospholipidswere taken at the beginning of the wheezing and at the onset of clinical remission.
Results. In patients of all groups there was an increase in the levels of phospholipids in EBC, in comparison with the control group. The increase in the levels was noted both at the onset of the disease and at the achievement of clinical remission. The highest levels of phospholipids in EBC were in children with asthma and in children who had more episodes of wheezing.
Conclusions.The study of the levels of OPL in CVI provides additional information on the state of the bronchopulmonary system and the degree of damage to cell membranes. This can be used to predict the progression of inflammation in the bronchopulmonary system.
Keywords: wheezing, bronchial asthma, children, exhaled breath condensate, phospholipids.


1. Antipkin YuG, Lapshin VF, Umanets TR, Zadorozhna TD, Pustovalova OI, Nakonechnalya AA. (2015). Inflammation markers and apoptosis of induced sputum cells in children with bronchial asthma and recurrent bronchitis. Journ NAMS of Ukraine. 21(1): 108-113.

2. Mizernitskiy YuL. (2014). Differential diagnosis and differential therapy of acute bronchial obstruction in acute respiratory viral infarction in young children. Practical medicine. 9(85): 82-88.

3. Odinets YuV, Ruchko AF, CherednIkova TYu. (2013). Possibilities of monitoring of acute bronchopulmonary diseases in children on the basis of analysis of exhaust air condensate. Child Health. 4(47): 44-48.

4. Ohotnikova EN. (2013). Pathogenetic features of bronchial obstructive syndrome in children and modern emergency care. Asthma and allergy. 2: 52-61.

5. ChernIsheva OE. (2014). Modern ideas about the pathogenesis of bronchial asthma in children. The health of the child. 5(56): 84-90.

6. Furman EG, Pechenkin YuM. (2014). Investigation of the condensation of exhaled air for respiratory diseases in children: reality and prospects. Perm Medical Journal. XXXI(2): 136-141.

7. Shipko AR. (2014). Actual questions of improvement of medical care for children with diseases of respiratory organs. Medicine for today and tomorrow. 1(62): 110-116.

8. Yakovleva OA. (2017). Markers of the condensate of exhaled air – the aspect of diagnosis and control of the effectiveness of pharmacotherapy. Rational pharmacotherapy. 4(45): 64-69.

9. Asher I, Pearce N. (2014). Global burden of asthma among children. Int J Tuberc Lung Dis. 18(11): 1269-1278. https://doi.org/10.5588/ijtld.14.0170; PMid:25299857

10. Benor S, Alcalay Y, Domany KA, Gut G, Soferman R, Kivity S, Fireman E. (2015). Ultrafine particle content in exhaled breath condensate in air ways of asthmatic children. J Breath Res. 9(2): 026001. https://doi.org/10.1088/1752-7155/9/2/026001.

11. Bodini A, Tenero L, Sandri M, Maffeis C, Piazza M, Zanoni L, Peroni D, Boner A, Piacentini G. (2017). Serum and exhaled breath condensate leptin levels in asthmatic and obesity children: a pilot study. J Breath Res. 11(4): 046005. https://doi.org/10.1088/1752-7163/aa61c5.

12. De Prins S, Marcucci F, Sensi L, Van de Mieroop E, Nelen V, Nawrot TS, Schoeters G, Koppen G. (2014). Exhaled nitric oxide and nasal tryptase are associated with wheeze, rhinitis and nasal allergy in primary school children. Epub. (6): 481-7. doi 10.3109/1354750X.2014.937362.

13. Klaassen EM, van de Kant KD, Jöbsis Q, van Schayck OC, Smolinska A, Dallinga JW, van Schooten FJ, den Hartog GJ, de Jongste JC, Rijkers GT, Dompeling E. (2015). Exhaled biomarkers and gene expression at preschool age improve asthma prediction at 6 years of age. Am J Respir Crit Care Med. 191(2): 201-7. https://doi.org/10.1164/rccm.201408-1537OC.

14. Ma H, Li Y, Tang L, Peng X, Jiang L, Wan J, Suo F, Zhang G, Luo Z. (2018). Impact of childhood wheezing on lung function in adulthood: A meta-analysis. Journal. pone: 0192390. eCollection. https://doi.org/10.1371/journal.pone.0192390

15. Yan DC, Chung FF, Lin SJ, Wan GH. (2016). The relationships among Dermatophagoides pteronyssinus exposure, exhaled nitric oxide, and exhaled breath condensate pH levels in atopic asthmatic children. Medicine (Baltimore). 95(39): e4825. https://doi.org/10.1097/MD.0000000000004825.