• Pecularities of the functional state of liver in children with inflammatory bowel diseases depending on variants of detoxification system gene polymorphism

Pecularities of the functional state of liver in children with inflammatory bowel diseases depending on variants of detoxification system gene polymorphism

PERINATOLOGIYA I PEDIATRIYA. 2017.2(70):134-139; doi 10.15574/PP.2017.70.134

Berezenko V. S., Tkalik E. N., Rossokha Z. I.
SI «Institute of Pedietrics, Obstetrics and Gynecology of NAMS of Ukraine», Kyiv
SI «Reference0centre for molecular diagnostic of the Ministry of Healthcare of Ukraine», Kyiv

Objective. To conduct research of the functional liver state features in children afflicted with inflammatory bowel diseases depending on detoxification system gene polymorphism (CYP2D6*4, GSTM1, GSTT1, MDR1).

Materials and methods. The study involved 44 children aged from 3 to 18, suffering from inflammatory bowel diseases. Functional liver state was estimated based on the main blood biochemical values, which characterise cytolytic, cholestatic and immunoinflammatory syndromes. The deletion polymorphism of GSTM1, GSTT1 genes was studied using the polymerase chain reaction, whereas the polymorphic variants of the CYP2D6*4(G1934A) and MDR1 (С3435Т) genes were detected by means of the polymerase chain reaction with the subsequent analysis of rstriction fragment length polymorphism.

Results. No reliable difference in frequency of polymorphic variants of studied genes was established in children with UC and CD. No correlation between functional liver state disorder and variants of first and second phase of detoxification genes polymorphism was found. Functional liver state disorder in form of cytolytic and immunoinflammatory syndromes was featured prominently in children with TT-genotype of the MDR1 gene.

Key words: children, inflammatory bowel diseases, functional liver state, detoxification genes.

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