- Activity of antimicrobial peptides and 25-hydroxycholecalciferol in children with asthma
Activity of antimicrobial peptides and 25-hydroxycholecalciferol in children with asthma
PERINATOLOGIYA I PEDIATRIYA. 2017.1(69):121-125; doi 10.15574/PP.2017.69.121
Activity of antimicrobial peptides and 25–hydroxycholecalciferol in children with asthma
Dudnyk V. М., Zaichko N. V., Fedchyshen О. P.
National Pirogov Memorial Medical University, Vinnytsya, Ukraine
Purpose — to determine the activity of antimicrobial peptides, such as cathelicidin LL-37 and 25-hydroxycholecalciferol in children with asthma.
Materials and methods. We have comprehensively examined 200 children with asthma aged 6 to 17 years. The contents of 25(OH)D3 and cathelicidin LL-37 in serum were determined by ELISA according to the instructions of the manufacturer.
Results. The examination revealed that the total content of 25(OH)D3 in the serum of children with asthma differs from the values of healthy children and characterized by a significant decrease of its level (p>0.01). Concentration of cathelicidin LL-37 in patients with asthma was significantly higher (p>0.001), than in the group of healthy children. The positive correlation between the cathelicidin LL-37, interleukin 1 (rxy=0.398 (p=0.02)) and interleukin 6 (rxy=0.178 (p=0.034)) in children with asthma was determined. The concentration of cathelicidin LL-37 in patients with optimal levels of 25(OH)D3 was significantly lower compared to children with deficiency of this vitamin (p<0.05).
Conclusions. The study found that the concentration of cathelicidin LL-37 in children with non-allergic asthma increased (1.87 times higher than with allergic form) and depended on the severity and control of the disease. It was established that the level of cathelicidin LL-37 in patients with 25(OH)D3 insufficiency 69.15% higher, than in patients with normal levels. The latter was due to the fact that 25(OH)D3 induces the regulatory T-cells, which potentially inhibit the production of pro-inflammatory interleukins.
Key words: asthma, children, 25-hydroxycholecalciferol, cathelicidin LL-37.
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